肝细胞癌中miR-942-5p的表达及其与患者恶性特征及不良预后的关系  

Expression of microRNA-942-5p in hepatocellular carcinoma and its relations with malignant feature and unfavorable outcomes of the patients

作　　者：丁琭 莫缓椰 鲜瑶[3] 涂康生[2] DING Lu;MO Huanye;XIAN Yao;TU Kangsheng(Department of Anesthesiology, the First Affiliated Hospital, Xian Jiaotong University, Xi'an 710061, China;Department of Hepatobiliary Surgery, the First Affiliated Hospital, Xian Jiaotong University, Xi'an 710061, China;Department of Nutrition, the First Affiliated Hospital, Xian Jiaotong University, Xi’an 710061, China)

机构地区：[1]西安交通大学第一附属医院麻醉科,陕西西安710061 [2]西安交通大学第一附属医院肝胆外科,陕西西安710061 [3]西安交通大学第一附属医院营养科,陕西西安710061

出　　处：《中国普通外科杂志》2019年第7期840-847,共8页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(81773123)

摘　　要：目的:探讨微小RNA-942-5p(miR-942-5p)在肝细胞癌(HCC)组织中的表达及其功能。方法:用实时定量PCR检测西安交通大学第一附属医院样本库保存的73例HCC组织和对应癌旁组织中miR-942-5p的表达。分析miR-942-5p表达与HCC患者临床病理资料的关系,同时分析TCGA数据库中miR-942-5p表达与HCC患者总生存率的关系。Transwell小室检测干扰miR-942-5p表达后HCC细胞迁移和侵袭能力的变化,StarBase V3.0网站和荧光素酶报告基因质粒预测分析miR-942-5p的下游靶点,并用Western blot验证。结果:miR-942-5p表达量在HCC组织中明显高于对应癌旁组织(2.390 vs. 1.764,P<0.05)。miR-942-5p表达量与HCC患者肿瘤数目、血管浸润和临床分期明显有关(均P<0.05)。miR-942-5p高表达HCC患者总生存率明显低于miR-942-5p低表达HCC患者(19.535%vs. 53.873%,P<0.05)。沉默miR-942-5p表达后,肝癌HCCLM3和MHCC97H细胞迁移和侵袭能力明显减弱(均P<0.05)。预测与分析结果显示,扣针蛋白5(FBLN5)是miR-942-5p的直接下游靶点(P<0.05),沉默miR-942-5p表达导致HCCLM3和MHCC97H细胞中FBLN5表达增加。结论:miR-942-5p在HCC组织中表达异常升高并与恶性临床特征和不良预后密切相关,机制可能与miR-942-5p抑制FBLN5表达促进HCC细胞迁移和侵袭有关。Objective: To investigate the expression of microRNA-942-5 p(miR-942-5 p) in hepatocellular carcinoma(HCC) tissue and its functions.Methods: The expressions of miR-942-5 p in 73 specimens of HCC tissue and paired tumor-adjacent tissue stored in the specimen bank of the First Affiliated Hospital, Xian Jiaotong University were determined by real-time quantitative PCR, and the relations of miR-942-5 p expression with clinicopathologic parameters of HCC patients were analyzed. Meanwhile, the relationship between miR-942-5 p expression and overall survival of HCC patients was identified by analyzing TCGA database. The changes in migration and invasion abilities of HCC cells after miR-942-5 p expression interference were observed by Transwell assay. The downstream target of miR-942-5 p was predicted and identified by using StarBase V3.0 database and Luciferase reporter assay, and then validated by Western blot. Results: The miR-942-5 p expression in HCC tissues was significantly higher than that in tumor-adjacent tissue(2.390 vs. 1.764, P<0.05). The expression level of miR-942-5 p was significantly associated with the number of lesions, vascular invasion and tumor stage of HCC patients(all P<0.05). The overall survival rate of HCC patients with high miR-942-5 p expression was significantly lower than HCC patients with low miR-942-5 p expression(19.535% vs. 53.873%, P<0.05). After silencing the miR-942-5 p expression, the migration and invasion abilities of liver cancer HCCLM3 and MHCC97 H cells were significantly weakened(both P<0.05). The results of prediction and analysis showed that fiblulin 5(FBLN5) was a direct downstream target of miR-942-5 p(P<0.05). Knockdown of the miR-942-5 p expression led to increased expression of FBLN5 in HCCLM3 and MHCC97 H cells.Conclusion: The miR-942-5 p expression is aberrantly up-regulated in HCC tissue and its overexpression is closely related to the malignant clinical features and poor prognosis. The mechanism may probably be responsible for miR-942-5 p inhibiting FBLN5 expression

关 键 词：癌 肝细胞 微RNAS 扣针蛋白5 肿瘤侵润 预后 

分 类 号：R735.7[医药卫生—肿瘤]