利用GEO数据集分析UBE2T在肺癌中的表达及临床意义  

Analysis of expression and its clinical significance of UBE2T in lung cancer based on GEO database

作　　者：李伟[1] 郭梓鑫 李永红[1] 刘小平[2] 黄静宇[2] 胡卫东[2] 李胜[3,4] LI Wei;GUO Zi-xing;LI Yong-hong;LIU Xiao-ping;HUANG Jing-yu;HU Wei-dong;LI Sheng(Department of Oncology,The First People s Hospital of Tianmen,Tianmen 431700,Hubei,China;Department of Thoracic Oncology,Zhongnan Hospital of Wuhan University,Wuhan University ,Wuhan 430071,Hubei,China;Department of Biological Repositories,Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei,China;Human Genetics Resource Preservation Center of Hubei Province,Wuhan 430071,Hubei,China)

机构地区：[1]湖北天门市第一人民医院肿瘤科,湖北天门431700 [2]武汉大学中南医院胸部肿瘤科,武汉430071 [3]武汉大学中南医院生物样本库,武汉430071 [4]湖北省人类遗传资源保藏中心,武汉430071

出　　处：《肿瘤代谢与营养电子杂志》2019年第2期242-245,共4页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：湖北省卫生计生科研基金(WJ2019H211);武汉大学珞珈青年学者科研基金(351人才计划)

摘　　要：目的探究UBE2T基因在肺癌中的表达情况及其与肺癌的临床病理特征和预后的关系。方法搜索NCBI的GEO数据库中的肺癌公共数据集,下载肺癌组织中的UBE2T表达数据和临床病理信息。分析UBE2T的表达与肺癌临床病理特征(年龄、T分期、N分期、M分期和病理分型)和复发、预后的关系。运用GSEA方法分析受UBE2T调控的基因。结果UBE2T在肺癌组织的表达水平高于正常组织的表达水平,差异有统计学意义(肺癌组织表达水平1.222±0.1214,正常组织表达水平-1.645±0.0783,P<0.05);肺癌组织UBE2T的表达与T分期、N分期、病理分型和复发有关(P<0.05),与性别、年龄和M分期无关(P>0.05)。UBE2T高表达组的5年总生存率和5年无病生存率分别为32.672%和27.513%,分别低于低表达组的66.767%和62.019%,差异有统计学意义(P<0.05)。GSEA结果显示UBE2T高表达样本富集了MYC信号通路、DNA修复因子、有丝分裂纺锤体、G2M检查点、mTORC1复合物、E2F转录因子和有丝分裂相关的基因集。结论UBE2T在肺癌组织中高表达,且与肺癌的多个临床病理特征有关,有潜力作为肺癌判断预后的标志物和治疗的靶向分子。Objective To investigate the expression of Ubiquitin-conjugating enzyme E2T(UBE2T)in lung cancer in the NCBI Gene Expression Omnibus(GEO)database and its relationship with clinicopathological characteristics and prognosis of lung cancer.Methods GEO datasets were collected and expression profile of UBE2T and clinicopathological information of lung cancer tissues were downloaded.The relationship between UBE2T expression and clinicopathological features(age,T stage,N stage,M stage,pathological type and recurrence)and prognosis of lung cancer were analyzed.Gene enrichment analysis(GSEA)was used to analyze genes regulated by UBE2T.Results The expression level of UBE2T in lung cancer tissues was 1.222±0.1214,higher than-1.645±0.0783 of normal bladder tissues,and the difference was statistically significant(P<0.05).The expression of UBE2T in lung cancer tissues was related to T stage,N stage,pathological analysis and recurrence(P<0.05),but not related to gender,age and M stage(P>0.05).The 5-year overall survival rate and 5-year disease-free survival rate of UBE2T high expression group were 32.672%and 27.513%,lower than 66.767%and 62.019%in the low-expression group,and the difference was statistically significant(P<0.05).GSEA results showed that UBE2T highly expressed samples enriched MYC signaling pathway,DNA repair factor,mitotic spindle,G2M checkpoint,mTORC1 complex,E2F transcription factor and mitotic related gene sets.Conclusion UBE2T is highly expressed in lung cancer tissues and is related to many clinicopathological features of lung cancer.UBE2T could be considered as a potential marker and target in the diagnosis and treatment of lung cancer.

关 键 词：肺癌 泛素结合酶E2T 基因表达汇编 临床意义 预后 

分 类 号：R734.2[医药卫生—肿瘤]