华蟾素联用顺铂对乳腺癌MDA-MB-231细胞活力、凋亡及周期分布的影响  

Effect of cinobufacini combined with Cisplatin on cell viability, apoptosis and cell cycle distribution of MDA-MB-231 cells

作　　者：肖聪 张懿敏[1] 孙圣荣[1] XIAO Cong;ZHANG Yimin;SUN Shengrong(Department of Breast and Thyroid Surgery,Renmin Hosptial of Wuhan University,Hubei Province,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院乳腺甲状腺外科

出　　处：《中国医药导报》2019年第21期12-16,共5页China Medical Herald

基　　金：国家自然科学基金青年科学基金项目(81502665)

摘　　要：目的研究华蟾素联用顺铂对乳腺癌MDA-MB-231细胞活力、凋亡和细胞周期分布的影响。方法体外培养乳腺癌MDA-MB-231细胞。CCK-8法检测华蟾素联用顺铂对细胞活力的影响;划痕实验检测华蟾素联用顺铂对细胞迁移能力的影响;流式细胞术分析华蟾素联用顺铂对细胞凋亡率及细胞周期分布的影响。结果CCK-8结果显示,与对照组比较,华蟾素组、顺铂组及华蟾素+顺铂组细胞活力降低,差异有统计学意义(P<0.05或P<0.01)。划痕结果显示,与对照组比较,华蟾素+顺铂组迁移能力显著下降,差异有高度统计学意义(P<0.01)。流式结果显示,与对照组比较,华蟾素+顺铂组G1期比例显著增多且凋亡更加明显,差异有统计学意义(P<0.05或P<0.01)。结论华蟾素联用顺铂能协同抑制乳腺癌MDA-MB-231细胞活力,可能与诱导细胞周期阻滞于G1期、抑制细胞迁移并促进细胞凋亡有关。Objective To study the effects of cinobufacini combined with Cisplatin on cell viability,apoptosis and cell cycle distribution of breast cancer MDA-MB-231 cells.Methods Breast cancer MDA-MB-231 cells were cultured in vitro.CCK-8 assay was used to detect the effect of cinobufacini combined with Cisplatin on cell viability.Scratch test was performed to detect the effect of cinobufacini combined with Cisplatin on cell migration.Flow cytometry was used to analyze the effects of cinobufacini combined with Cisplatin on the apoptosis rate and cell cycle distribution of breast cancer cells.Results CCK-8 results showed that compared with the control group,the cell viability in the cinobufacini group,the Cisplatin group and the cinobufacini+Cisplatin group was decreased,the differences were statistically significant(P<0.05 or P<0.01).The scratch results showed that the migration ability of the cinobufacini+Cisplatin group was significantly decreased compared with the control group,the difference was highly statistically significant(P<0.01).Flow cytometry showed that compared with the control group,the proportion of G1 phase in the cinobufacini+Cisplatin group increased significantly and the apoptosis was more obvious,the differences were statistically significant(P<0.05 or P<0.01).Conclusion Cinobufacini combined with Cisplatin shows a synergic effect in inhibiting MDA-MB-231 cells,which may be related to the induction of cell cycle arrest at G1 phase,inhibition of cell migration and promotion of cell apoptosis.

关 键 词：华蟾素 顺铂 乳腺癌 MDA-MB-231细胞 

分 类 号：R737.9[医药卫生—肿瘤]