前列腺癌细胞系中环状RNA circRNA-1565的表达及鉴定  

Expression and Identification of Ring RNA CircRNA-1565 in Prostate Cancer Cell Line

作　　者：时浩清 訾晓渊 张春雷 杨琦 孙颖浩 SHI Hao-qing;ZI Xiao-yuan;ZHANG Chun-lei;YANG Qi;SUN Ying-hao(Department of Urology,Shanghai Changhai Hospital,Naval Military Medical University,Shanghai,200433,China)

机构地区：[1]海军军医大学长海医院泌尿外科

出　　处：《现代生物医学进展》2019年第12期2243-2247,共5页Progress in Modern Biomedicine

基　　金：上海市浦江人才计划项目(16PJ1411100)

摘　　要：目的:探讨前列腺癌细胞系中的环状RNA circRNA-1565的表达及鉴定。方法:培养前列腺正常上皮细胞(RWPE-1)、4种前列腺癌细胞(22RV1、LNCap、PC-3、DU145),提取总RNA,采用RT-PCR检测不同细胞系中circRNA-1565的表达,并对其进行成环性验证及细胞内亚定位实验,进行差异比较。结果:circRNA-1565在正常前列腺细胞系(RWPE-1)中表达量极低,在转移性前列腺癌细胞系中高表达,在非转移性前列腺癌细胞系中低表达。且circRNA-1565是一条主要定位于细胞质内的具有有效环状结构的RNA分子。结论:circRNA-1565在不同前列腺癌细胞系中存在差异表达,可能会通过mi RNA sponge途径发挥生物学作用。Objective: To investigate the expression and identification of annular RNA circRNA-1565 in the cell line of prostate cancer. Methods: Normal prostate epithelial cells(RWPE-1), 4 kinds of prostate cancer cells(22 RV1, LNCap, PC-3, DU145) were cultured, total RNA was extracted, and RT-PCR was used to detect the expression of circRNA-1565 in different cell lines. The cyclic verification and intracellular localization experiments were carried out to compare the differences. Results: The expression of circRNA-1565 in normal prostate cell line(RWPE-1) was very low, expressed highly in metastatic prostate cancer cell line, and low in non-metastatic prostate cancer cell line. And circRNA-1565 is a RNA molecule with an effective annular structure, which is mainly located in the cytoplasm. Conclusion: The expression of circRNA-1565 in different prostate cancer cell lines may play a biological role through the sponge pathway of mi RNA.

关 键 词：前列腺癌 环状RNA circRNA 基因表达 

分 类 号：R-33[医药卫生] R737.25