丹参酮ⅡA与人肝癌细胞HepG2体内外增殖和凋亡的相关性  

Correlation between tanshinone ⅡA and proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 in vitro and in vivo

作　　者：袁建华 李伟学[1] 田宋君[1] YUAN Jian-hua;LI Wei-xue;TIAN Song-jun(Department of General Surgery, Hanyang Hospital Affiliated to Wuhan University of Science and Technology ,Wuhan 430051, China)

机构地区：[1]武汉科技大学附属汉阳医院普外科

出　　处：《中国现代普通外科进展》2019年第7期505-509,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨丹参酮ⅡA对人肝癌细胞HepG2体内外增殖、凋亡相关研究。方法:采用不同浓度(0、1、2、4、8μg/mL)、不同时间(24、48、72h)丹参酮ⅡA处理人肝癌HepG2细胞,采用MTT法检测人肝癌细胞HepG2增殖抑制率,采用流式细胞仪技术检测细胞凋亡情况,采用Westernlot检测Bax、Bcl-2蛋白表达;建立裸鼠肝癌模型,测量给药1、6、12和21d肿瘤体积变化。结果:丹参酮ⅡA作用24、48、72h对HepG2细胞抑制率逐渐升高,差异有统计学意义(P<0.05),且呈一定的药物剂量依赖性(P<0.05)。采用AnnexinV-FITC/PI双染法检测各浓度丹参酮ⅡA对HepG2细胞凋亡的影响,各剂量丹参酮ⅡA的细胞凋亡率明显高于对照组(P<0.05),且随着丹参酮ⅡA浓度增加,细胞凋亡率明显升高(P<0.05)。各丹参酮ⅡA药物组Bcl-2表达均低于对照组(P<0.05),且随着丹参酮ⅡA药物浓度的升高,Bcl-2表达明显降低(P<0.05);各丹参酮ⅡA药物组Bax表达均高于对照组(P<0.05),且随着丹参酮ⅡA药物浓度的升高,Bax表达明显升高(P<0.05)。丹参酮ⅡA低剂量组和丹参酮ⅡA高剂量组给药12、21d肿瘤体积低于同期对照组,且丹参酮ⅡA高剂量组低于丹参酮ⅡA低剂量组,差异均有统计学意义(P<0.05)。结论:丹参酮ⅡA可有效抑制肝癌HepG2细胞增殖,促进细胞凋亡,且其作用与丹参酮ⅡA药物浓度相关,丹参酮ⅡA可能通过调节凋亡相关基因Bcl-2、Bax表达可能是其促进细胞凋亡及抑制肿瘤体积增长。Objective:To investigate the study of tanshinone ⅡA on proliferation and apoptosis of human hepatocellular carcinoma cell HepG2 in vitro and in vitro. Methods:The human hepatocellular carcinoma HepG2 cells were treated with different concentrations(0, 1, 2, 4, 8 μg/mL) and different time (24, 48, 72 h) tanshinone ⅡA. The proliferation inhibition rate of human hepatocellular carcinoma was detected by MTT, and the apoptosis was detected by flow cytometry. The expression of Bax and Bcl-2 proteins was detected by Western lot, and the liver of nude mice was established. The tumor size was measured by 1d, 6d, 12d and 21d. Results:The inhibitory rate of tanshinone II A on HepG2 cells increased gradually after 24, 48 and 72 hours(P<0.05), and showed a dose-dependent manner(P<0.05). Annexin V-FITC/PI double staining method was used to detect the effect of tanshinone II A on apoptosis of HepG2 cells. The apoptotic rate of HepG2 cells was significantly higher than that of the control group(P<0.05), and the apoptotic rate increased significantly with the increase of tanshinone II A concentration(P<0.05). The expression of Bcl-2 in tanshinone II A group was lower than that in control group(P<0.05), and the expression of Bcl-2 decreased significantly with the increase of tanshinone II A concentration(P<0.05);the expression of Bax in tanshinone II A group was higher than that in control group(P<0.05), and the expression of Bax increased significantly with the increase of tanshinone II A concentration(P<0.05). Tumor volume of each group increased 12 and 21 days after administration compared with 6 days after administration, and increased 21 days after administration compared with 12 days after administration(P<0.05). Tumor volume of low dose tanshinone II A group and high dose tanshinone II A group on 12 and 21 days after administration was lower than that of control group, and the high dose tanshinone II A group was lower than that of low dose tanshinone II A group, with statistical significance (P<0.05). Conclusion:tanshi

关 键 词：丹参酮ⅡA 肝癌HEPG2细胞 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R737.7[医药卫生—肿瘤]