Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus  

作　　者：DU Guangxun QU Lingyun SHANG Kun SUN Chengjun WANG Chen GAO Ping 

机构地区：[1]First Institute of Oceanography,Ministry of Natural Resources (MNR),Qingdao 266061,China [2]Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China [3]College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China

出　　处：《Journal of Oceanology and Limnology》2019年第5期1726-1735,共10页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2017YFC1404504);the National Basic Research Program of China(973 Program)(No.2015CB755904);the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2016ASKJ14);the Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology(No.2016LMFS-B08)

摘　　要：To identify the pathogen that causes scuticociliatosis in farmed-raised turbot Scophthalmus maximus , we isolated a ciliate from the brain tissue of an infected turbot and identifi ed it as Uronema marinum based on morphological and molecular evidence. We then infected the turbots in artifi cial laboratory settings with pure cultured U . marinum . The infected turbots showed syndromes similar to those observed in naturally infected ones. Furthermore, microscopic examination and PCR detection confi rmed the presence of the ciliate in the tissues of those laboratory-infected turbots. To our best knowledge, this is the fi rst report of scuticociliatosis caused by U . marinum in farm-raised turbot S . maximus in China.To identify the pathogen that causes scuticociliatosis in farmed-raised turbot Scophthalmus maximus,we isolated a ciliate from the brain tissue of an infected turbot and identified it as Uronema marinum based on morphological and molecular evidence.We then infected the turbots in artificial laboratory settings with pure cultured U.marinum.The infected turbots showed syndromes similar to those observed in naturally infected ones.Furthermore,microscopic examination and PCR detection confirmed the presence of the ciliate in the tissues of those laboratory-infected turbots.To our best knowledge,this is the first report of scuticociliatosis caused by U.marinum in farm-raised turbot S.maximus in China.

关 键 词：Scophthalmus MAXIMUS scuticociliatosis HISTOPATHOLOGY Uronema marinum 

分 类 号：P[天文地球]