蜗牛匀浆液直肠给药对溃疡性结肠炎大鼠的保护作用  

Protective effect of snail homogenate enema on ulcerative colitis in rats

作　　者：范志源 余秋雨 李欢 苏小娇 盛艳梅[1] FAN Zhiyuan;YU Qiuyu;LI Huan;SU Xiaojiao;SHENG Yanmei(School of Pharmacy,Chengdu Medical College,Sichuan Province,Chengdu 610500,China)

机构地区：[1]成都医学院药学院

出　　处：《中国医药导报》2019年第22期17-20,25,共5页China Medical Herald

基　　金：四川省大学生创新创业项目(201613705021)

摘　　要：目的探讨蜗牛匀浆液直肠给药对溃疡性结肠炎大鼠是否具有保护作用。方法用冰醋酸诱导急性溃疡性结肠炎大鼠模型,将40只6~7周龄的SPF级雄性SD大鼠采用随机数字表法分为模型组、空白组、蜗牛匀浆液的高剂量组(1.0g/kg)和低剂量组(0.5g/kg),每组各10只。造模后,高、低剂量组连续直肠给予蜗牛匀浆液7d,空白组与模型组均给予相同体积的生理盐水。每日观察大鼠的主要体征与粪便性状,同时计算体重变化并进行粪便评分。实验结束后,处死大鼠取目标结肠段,称量计算肠重系数,同时采用HE染色法进行病理组织检测。结果与模型组比较,高剂量组和低剂量组大鼠体征明显改善,大鼠体重增加程度更显著(P<0.05或P<0.01),并且肠重系数显著降低(P<0.01),高剂量组给药后第4天开始出现粪便评分显著下降(P<0.05或P<0.01)。同时,高剂量组炎性细胞浸润及炎症细胞浸润深度评分和溃疡深度评分与模型组比较,差异有高度统计学意义(P<0.01)。结论蜗牛匀浆液对溃疡性结肠炎模型大鼠可通过改善体征、增加体重、降低腹泻、对抗结肠组织水肿及炎性反应等产生较好的保护作用,尤以高剂量组作用明显。Objective To investigate whether snail homogenate has protective effect on ulcerative colitis in rats.Methods Rat model of acute Ulcerative Colitis induced by glacial acetic acid.Forty male Sprague-Dawley rats aged 6-7 weeks were randomly divided into model group,blank group,snail homogenate high (1.0 g/kg) and low (0.5 g/kg) dose groups according to random number table,with 10 rats in each group.After the model was completed,the high (1.0 g/kg) and low (0.5 g/kg) dose groups were given snail homogenate continuously for 7 days in the rectum,and the blank group and the model group were given the same volume of normal saline.The main signs and fecal traits of the rats were observed daily,and body weight changes were calculated and fecal scores were performed.At the end of the experiment,the rats were sacrificed and the target colon segment was taken.The coefficient of intestinal weight was calculated and the pathological tissue was detected by HE staining.Results Compared with model group,the symptoms in rats of high-dose group and low-dose group were significantly improved,the degree of weight gain of rats increased significantly (P < 0.05 or P < 0.01),and intestinal weighting coefficient reduced significantly (P < 0.05 or P < 0.01),a significant decrease in fecal scores of high-dose group began on day 4 after administration (P < 0.01).Meanwhile,the scores of inflammatory cell infiltration depth and ulcer depth in the high-dose group were significantly different from those in the model group (P < 0.01).Conclusion Snail homogenate can improve the physical signs,increase body weight,reduce diarrhea,and fight colonic hyperplasia and inflammatory reaction in rats with ulcerative colitis,especially at high doses.

关 键 词：蜗牛匀浆液 溃疡性结肠炎 直肠给药 保护作用 

分 类 号：R574.62[医药卫生—消化系统]