Determination of Genistin Content in Flemingia philippinensis Merr. et Rolfe by High Performance Liquid Chromatography (HPLC)  

作　　者：Xiaoqing Wang Huiying Zhang 

机构地区：[1]Qujing Medical College,Qujing,Yunnan,655000

出　　处：《Journal of Medicines Development Sciences》2018年第1期27-30,共4页药物发展科学期刊

摘　　要：To set up the HPLC method in determination of the genistin content in Flemingia philippinensis Merr.et Rolfe.Method: Extract and filter the crushed Flemingia philippinensis Merr.et Rolfe that has been soaked in methanol,the filtrate of which is to be taken out as the sample.Shimadzu LC-2010A is adopted in this experiment which is performed on Agilent Extend-RP column with acetonitrile-0.1% phosphoric acid aqueous solution (19:81) as mobile phase.The flow rate is 1.0 mL/min and the UV detective wavelength is 262 nm.Result: The linear relationship is excellent with higher recovery rate within the range.This method is rapid,simple and accurate.

关 键 词：GENISTIN FLEMINGIA philippinensis Merr. ET Rolfe High Performance Liquid Chromatography (HPLC) 

分 类 号：R[医药卫生]