高胆固醇饮食对斑马鱼脂代谢的影响  

Effects of high cholesterol diet on the lipid metabolism in zebrafish larvae

作　　者：武琳 陈忠[2] 余翠 刘帅[3] 杨明[3] Wu Lin;Chen Zhong;Yu Cui;Liu Shuai;Yang Ming(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306;Department of Cardiology,Shanghai Sixth People' s Hospital East Affiliated to Shanghai University of Medicine and Health Sciences,Shanghai 201306;College of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海健康医学院附属第六人民医院东院(上海市第六人民医院东院)心内科,上海201306 [3]上海大学环境与化工学院,上海200444

出　　处：《南京医科大学学报（自然科学版）》2019年第7期978-982,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：上海健康医学院师资人才项目

摘　　要：目的:建立一种稳定可靠的斑马鱼高脂血症模型,并应用不同方法评估高胆固醇饮食对斑马鱼脂代谢的影响。方法:受精5 d后(5 days post fertilization,5dpf)的斑马鱼幼鱼随机分成普通饲料组和高胆固醇饲料模型组,普通饲料为纯蛋黄干粉,高胆固醇饲料是向纯蛋黄干粉中添加不同百分比的胆固醇配制而成,连续喂养10 d后通过显微镜实时观察并对幼鱼生理生化指标进行检测评估。结果:共聚焦显微镜下观察到不同百分比的高胆固醇饮食模型组幼鱼尾部动脉血管脂质沉积量均显著高于普通饲料组(P <0.05),且随着饲料中胆固醇百分比增加,幼鱼动脉血管脂质沉积量也随之增加;增加5%高胆固醇饮食组投喂量,其血管脂质沉积量先显著性增加(P <0.05)后渐趋稳定,但幼鱼生存率逐渐降低(P <0.05);5%高胆固醇饮食组幼鱼体宽明显大于普通饲料组(P <0.01),但体长无明显差异(P> 0.05);5%高胆固醇饮食组幼鱼体内总胆固醇(total cholesterol,TC)和总甘油三酯(total triglyceride,TG)水平较普通饲料组明显升高(P <0.01),且低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)水平也同时升高(P <0.05)。结论:5%高胆固醇饲料喂养10 d可以诱导斑马鱼高脂血症的发生,且适量增加饲料中胆固醇百分比或适当增加高胆固醇投喂量均能加快高脂血症模型建立。Objective:To establish a stable and reliable model of zebrafish hyperlipidemia and evaluate the effect of high cholesterol diet on the lipid metabolism in zebrafish larvae. Methods:Zebrafish larvae after five days post fertilization(5 dpf)were randomly divided into two groups:normal feed group with dry powder of pure egg yolk,and high cholesterol feed model group with different percentages of cholesterol added to pure egg yolk. After 10 days,the evaluation was carried out by microscopy and detecting the physiological and biochemical indicators in the zebrafish larvae. Results:Confocal microscopy showed that the lipid deposition in caudal artery of juvenile fishes in high cholesterol diet model group was significantly higher than that in common diet group(P < 0.05),and the lipid deposition in caudal artery of juvenile fishes increased with the increase of cholesterol content in diet. When the feed intake of the 5% high cholesterol diet group increased,the lipid deposition in blood vessel increased significantly at the beginning(P <0.05)and subsequently stabilized gradually,but the survival rate of juvenile fishes decreased significantly(P < 0.05). The body width of fish larvae in the 5% high cholesterol diet group was significantly larger than that in common diet group(P < 0.01),but no significant differences in body length were observed between the two groups(P > 0.05). The levels of total cholesterol(TC)and total triglyceride(TG)in juvenile fishes fed with 5% high cholesterol diet were both significantly higher than those fed with normal diet(P <0.01),and the levels of LDL-C were also significantly higher(P <0.05). Conclusion:Feeding 5% high cholesterol diet for 10 days could induce the occurrence of hyperlipidemia in zebrafish. Increasing the percentage of cholesterol in diet or increasing the dosage of high cholesterol properly could accelerate the establishment of hyperlipidemia model.

关 键 词：胆固醇 高血脂 斑马鱼 模型 蛋黄 

分 类 号：R589.2[医药卫生—内分泌]