长链非编码RNA AL451105.2对前列腺癌细胞增殖和侵袭的影响  

Effect and mechanism of long-chain non-coding RNA AL451105.2 on proliferation and invasion of prostate cancer cells

作　　者：程树林[1] 蔡涛 周文浩[1] 陈双全[1] 朱平宇[1] CHENG Shulin;CAI Tao;ZHOU Wenhao;CHEN Shuangquan;ZHU Pingyu(Department of Urology,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院泌尿外科

出　　处：《实用医学杂志》2019年第15期2389-2393,共5页The Journal of Practical Medicine

基　　金：四川省卫生和生育计划委员会基金(编号:18PJ456);川北医学院校级课题基金(编号:CBY16-A-YB16)

摘　　要：目的探讨长链非编码RNA AL451105.2在前列腺癌组织和细胞中的表达,并观察其对前列腺癌细胞增殖和侵袭的影响。方法荧光实时定量聚合酶链反应(qPCR)检测16例前列腺癌组织及癌旁组织、前列腺癌细胞株和正常前列腺上皮细胞中AL451105.2的表达水平。以表达水平最低的前列腺癌细胞为转染对象,转染对照质粒(对照组)或转染含有AL451105.2全长的质粒(实验组)。生物信息学预测AL451105.2的下游基因。qPCR检测转染后细胞中AL451105.2和下游基因的表达;Western blot检测下游蛋白的表达;细胞计数试剂盒(CCK-8法)和Transwell实验分别检测AL451105.2对前列腺癌细胞增殖和侵袭能力的影响。结果 AL451105.2在前列腺癌组织中的表达明显低于癌旁组织(P <0.01),在前列腺癌细胞株中的表达明显低于正常前列腺上皮细胞(P <0.05),在DU-145细胞中表达量最低(P <0.01)。AL451105.2可靶向结合miR-181a-5p,miR-181a-5p可靶向结合锌指268(ZNF268)。AL451105.2在实验组细胞中的表达明显高于对照组(P <0.01)。miR-181a-5p在实验组细胞中的表达明显低于对照组(P <0.01)。ZNF268在mRNA水平和蛋白水平的表达明显升高(P <0.05)。高表达AL451105.2的细胞增殖能力明显降低(P <0.05),细胞侵袭能力明显降低(P <0.01)。结论 AL451105.2在前列腺癌组织和细胞中低表达,高表达AL451105.2可明显抑制前列腺癌细胞的增殖和侵袭能力,其可能的分子机制是抑制miR-181a-5p的表达,促进ZNF268基因的表达。AL451105.2低表达可能是调控前列腺癌发生发展的重要分子机制。Objective To investigate the expression of AL451105.2 in prostate cancer tissues and cells,and to observe its effect on proliferation and invasion of prostate cancer cells. Methods Fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression level of AL451105.2 in 16 prostate cancer tissues and adjacent tissues,prostate cancer cells and normal bladder epithelial cells. The prostate cancer cells with the lowest expression level were transfected with the control plasmid(control group)or the plasmid containing the full length of AL451105.2(experimental group). Bioinformatics predicts downstream genes for AL451105.2. QPCR was used to detect the expression of AL451105.2 and downstream genes in transfected cells;western blotting was used to detect the expression of downstream proteins;cell counting kit(CCK-8 method)and transwell assay were used to detect the effects of AL451105.2 on the proliferation and invasiveness of prostate cancer cells. Results The expression of AL451105.2 in prostate cancer tissues was significantly lower than that in adjacent tissues(P < 0.01),and the expression in prostate cancer cell lines was significantly lower than that in normal bladder epithelial cells(P < 0.05),and the expression level was lowest in the DU-145 cells(P < 0.01). AL451105.2 can bind to miR-181 a-5 p,and miR-181 a-5 p can bind to zinc finger 268(ZNF268). The expression of AL451105.2 in the experimental group was significantly higher than that in the control group(P < 0.01). The expression of miR-181 a-5 p in the experimental group was significantly lower than that in the control group(P < 0.01). The expression of ZNF268 was significantly increased at mRNA and protein levels(P < 0.05). After transfected with AL451105.2,the cell proliferation ability and the cell invasion ability were significantly decreased(P < 0.01). Conclusions AL451105.2 is lowly expressed in prostate cancer tissues and cells,and high expression of AL451105.2 can significantly inhibit the proliferation and invasion of prostat

关 键 词：前列腺癌 长链非编码RNA miR-181a-5p 锌指268 

分 类 号：R737.25[医药卫生—肿瘤]