氧化葡萄糖酸杆菌中硫辛酸合成模块对维生素C一步混菌发酵的影响  

Biosynthesis of a-lipoic acid in Gluconobacter oxydans increases the production of vitamin C by one-step fermentation

作　　者：刘宇 王恩旭[1,2] 潘才惠 董秀涛[1,2] 丁明珠 Yu Liu;Enxu Wang;Caihui Pan;Xiutao Dong;Mingzhu Ding(Key Laboratory of Systems Bioengineering (Ministry of Education),Frontier Science Center for Synthetic Biology,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;Collaborative Innovation Center of Chemical Science and Engineering (Tianjin),Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学化工学院教育部合成生物学前沿科学中心系统生物工程教育部重点实验室,天津300072 [2]天津大学天津化学化工协同创新中心,天津300072

出　　处：《生物工程学报》2019年第7期1266-1276,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.21676190)资助~~

摘　　要：在由氧化葡萄糖酸杆菌和普通生酮古龙酸杆菌构建的维生素C两菌一步发酵体系中,为了强化氧化葡萄糖酸杆菌对普通生酮古龙酸杆菌生长和产酸的促进作用,文中在氧化葡萄糖酸杆菌中构建硫辛酸合成功能模块。由含硫辛酸功能模块的氧化葡萄糖酸杆菌和普通生酮古龙酸杆菌组成的两菌一步体系,能减轻普通生酮古龙酸杆菌单菌培养时的生长抑制,强化两菌的互作关系,使维生素C前体(2-酮基-L-古龙酸,2-KGA)的产量提高到73.34 g/L(对照组为59.09 g/L),醇酸转化率提高到86.0%。研究结果为进一步优化维生素C两菌一步发酵体系提供了新思路。In a one-step fermentation system of vitamin C production with Gluconobacter oxydans and Ketogulonicigenium vulgare,a functional module of α-lipoic acid biosynthesis was constructed in G.oxydans.The engineered G.oxydans was co-cultured with K.vulgare to enhance the growth and 2-keto-L-gulonic acid(2-KGA)production of K.vulgare.This one-step fermentation system alleviated the growth inhibition during the mono-culture of K.vulgare and strengthened the interaction between the two bacteria.Moreover,the yield of vitamin C precursor(2-KGA)increased to 73.34 g/L(the control group was 59.09 g/L),and the conversion of D-sorbitol to 2-KGA increased to 86.0%.This study provides a new idea for further optimizing the one-step fermentation system of vitamin C production.

关 键 词：一步发酵 氧化葡萄糖酸杆菌 普通生酮古龙酸杆菌 硫辛酸 

分 类 号：TQ924[轻工技术与工程—发酵工程]