口腔扁平苔藓活化成纤维细胞对血管内皮细胞迁移及成管特性的影响  

Effect of Oral Lichen Planus-associated Myofibroblasts on Migration and Tube-formation of Vascular Endothelial Cells

作　　者：孟文霞 冯璐 刘曙光 周会喜 李志强 MENG Wenxia;FENG Lu;LIU Shuguang;ZHOU Huixi;LI Zhiqiang(Department of Oral Mucosa,Stomatological Hospital,Southern Medical University,Guangzhou 510280,China;Department of Oral and Maxillofacial Surgery,Stomatological Hospital,Southern Medical University,Guangzhou 510280,China)

机构地区：[1]南方医科大学口腔医院黏膜科,广东广州510280 [2]南方医科大学口腔医院颌面外科,广东广州510280

出　　处：《口腔医学研究》2019年第8期806-809,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81500850);国家卫生计生委公益性行业专项(编号:201502018)

摘　　要：目的:研究体外分离培养的原代扁平苔藓活化成纤维细胞(oral lichen planus-associated myofibroblasts,OLP-MFs )对血管内皮细胞迁移和成管特性的影响,探讨扁平苔藓炎性微环境中OLP-MFs对扁平苔藓病损血管生成的作用。方法:取充血糜烂型口腔扁平苔藓患者颊部组织,采用组织块原代分离培养方法获取原代成纤维细胞,第3代细胞进行表型鉴定,以获取α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)阳性表达的OLP-MFs。以口腔黏膜正常成纤维细胞(normal fibroblasts,NFs)和未经处理的人脐静脉血管内皮细胞(human vascular endothelial cells,HUVEC)为对照组,采用Transwell迁移实验观察OLP-MFs与HUVEC间接共培养过程中对血管内皮细胞的迁移;成管实验观察OLP-MFs对HUVEC形成血管的影响。结果:与NFs相比,OLP-MFs细胞高表达α-SMA,具有肌成纤维细胞的特性。以HUVEC对照组迁移细胞数为100%计算,NFs+HUVEC组迁移细胞百分比为(114.86±8.02)%( P >0.05),OLP-MFs+HUVEC组迁移细胞数百分比为(169.83±7.58)%( P <0.05),OLP-MFs与NFs相比明显促进了HUVEC的迁移。以HUVEC对照组形成管长度为100%计算,对照组NFs+HUVEC形成管长度百分率为(100.38±10.54)%,OLP-MFs+HUVEC组形成成管长度百分率为(159.90±12.98)%,OLP-MFs+HUVEC组和NFs+HUVEC组比较差异有统计学意义( P <0.01)。结论:口腔扁平苔藓组织微环境中存在具有活化特性的成纤维细胞,且能明显促进HUVEC迁移和成血管分化。Objective:To observe the effect of oral lichen planus (OLP)-associated myofibroblasts (MFs) on the migration and tube-formation of vascular endothelial cells,and to elucidate the role of OLP-MFs in the angiogenesis of oral lichen lesions.Methods:Tissues were obtained from the OLP patients.The specimens were analyzed using the primary separation culture methods.Cultures at passage three were used for the cellular identification of OLP-MFs.Normal fibroblasts and untreated HUVEC were set as the control group.Transwell cell migration assay was used to detect the migration of OLP-MFs after co-cultured with HUVEC.Endothelial tube formation assay was used to detect the tube formation of OLP-MFs.Results:Compared with the control groups,OLP-MFs cells highly expressed α-SMA and had the related characteristics of myofibroblasts.Considered the migration cell number was 100% in HUVEC group,NFs + HUVEC group was (114.86±8.02)%,OLP-MFs + HUVEC group was (169.83 ±7.58)%,and OLP-MFs significantly promoted the migration of HUVEC.The tube length of OLP-MFs + HUVEC was (159.90±12.98)%,and the control groups were (100.38±10.54)% and 100%,respectively.Conclusion:The activated fibroblasts exist in the microenvironment of oral lichen planus,and can significantly pro- mote HUVEC migration and tube length formation.

关 键 词：口腔扁平苔藓 活化成纤维细胞 血管内皮细胞 迁移 血管形成 

分 类 号：R781.5[医药卫生—口腔医学]