BRAFV600E/端粒酶反转录酶在甲状腺乳头状癌肺转移中的表达及其临床意义  

BRAFV600E/telomerase reverse transcriptase protein expression in lung metastasis of papillary thyroid carcinoma and clinical significance

作　　者：陈玲[1] 朱有志[1] 郑珂[2] 张惠灏[1] 吴坤琳[1] 孔令君[1] 林伟[1] 莫彩琴 薛佳杰 陈祥锦[1] Chen Ling;Zhu Youzhi;Zheng Ke;Zhang Huihao;Wu Kunlin;Kong Lingjun;Lin Wei;Mo Caiqin;Xue Jiajie;Chen Xiangjin(Department of Thyroid and Breast Surgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350009, China;Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350009, China)

机构地区：[1]福建医科大学附属第一医院甲状腺乳腺外科,福州350009 [2]福建医科大学附属第一医院病理科,福州350009

出　　处：《中华实验外科杂志》2019年第8期1455-1458,共4页Chinese Journal of Experimental Surgery

基　　金：国家临床重点专科建设项目(国卫办医函[2013]544号).

摘　　要：目的探讨BRAFV600E/端粒酶反转录酶(TE RT)在甲状腺乳头状癌(PTC)患者中的蛋白表达及与肺转移的相关性.方法收集716例PTC患者的癌组织,用免疫组织化学法检测BRAFV600E、TERT表达,并结合其临床病理学特点进行分析.结果在PTC肿瘤组织中,62.3%(446/716)存在BRAFV600E蛋白表达,30.9%(221/716)存在TERT蛋白表达,18%(129/716)的患者同时检测到BRAFV600E及TERT蛋白表达.多因素分析表明,特殊亚型乳头状癌、肿瘤最大径、侧颈区淋巴结转移以及BRAFV600E/TERT蛋白双表达是PTC发生肺转移的独立因素;且肿瘤最大径、侧颈区淋巴结转移以及BRAFV600E/TERT蛋白双表达在预测PTC患者发生肺转移具有较高的诊断准确性[曲线下面积(AUC)>0.7].结论特殊亚型乳头状癌、肿瘤最大径、侧颈区淋巴结转移和BRAFV600E/TERT蛋白双表达是PTC发生肺转移的独立危险因素.Objective To investigate the protein expression of BRAFV600E/telomerase reverse transcriptase (TERT) in patients with papillary thyroid cancer (PTC) and its correlation with lung metastasis. Methods The tumor tissues of 716 patients with PTC were collected. The expression of BRAFV600E and TERT was detected by immunohistochemistry, and their clinical pathological features were analyzed. Results In tumor tissues of these PTC patients, 62.3%(446/716) expressed BRAFV600E protein, 30.9%(221/716) expressed TERT protein, and 18%(129/716) co-expressed BRAFV600E and TERT proteins. Multivariate analysis revealed that special subtype of papillary carcinoma, maximal diameter of tumor, lateral cervical lymph node metastasis, and co-expression of BRAFV600E/TERT proteins were independent risk factors of PTC lung metastasis. Tumor maximum diameter, lateral cervical lymph node metastasis and co-expression of BRAFV600E/TERT proteins had a high diagnostic accuracy [area under curve (AUC)>0.7] in predicting lung metastasis in PTC patients. Conclusion The specific subtype of papillary carcinoma, tumor maximum diameter, lateral cervical lymph node metastasis and co-expression of BRAFV600E/TERT proteins were independent risk factors for PTC pulmonary metastasis.

关 键 词：甲状腺乳头状癌 肺转移 BRAFV600E 端粒酶反转录酶 

分 类 号：R736.1[医药卫生—肿瘤]