B细胞转位基因3在乳腺癌组织的表达及其对乳腺癌细胞增殖和侵袭的影响  

Expression of B cell translocation gene 3 in breast cancer tissues and its effect on proliferation and invasion of breast cancer cells

作　　者：王文慧 邓甬川[1] 陈豪 温媛媛[3] Wang Wenhui;Deng Yongchuan;Chen Hao;Wen Yuanyuan(Department of Surgical Oncology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China;Department of Pathology, Hangzhou Obstetrics and Gynecology Hospital, Hangzhou 310008, China;Department of Pathology, Zhejiang Zhoushan Hospital, Zhoushan 316021, China)

机构地区：[1]浙江大学医学院附属第二医院肿瘤外科,杭州310009 [2]杭州市妇产科医院病理科,310008 [3]浙江省舟山医院病理诊断中心,316021 [4]杭州市妇产科医院,310008

出　　处：《中华实验外科杂志》2019年第8期1462-1465,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察B细胞转位基因3(BTG3)在乳腺癌组织中的表达及其对乳腺癌细胞增殖和侵袭的影响.方法选择80例乳腺癌患者的乳腺癌组织和癌旁组织,采用蛋白质印迹法(Western blot)和反转录-聚合酶链反应(RT-PCR)测定乳腺癌组织和癌旁组织中BTG3蛋白和mRNA水平.将MCF-7细胞分为Blank组(不转染)、NC组(转染空载体)和Ex-BTG3组(转染过表达BTG3载体).噻唑蓝(MTT)法测定细胞增殖能力,Transwell小室测定细胞侵袭能力,划痕实验测定细胞迁移能力,Western blot和RT-PCR测定细胞BTG3、转化生长因子-β1(TGF-β1)、人信号转导分子7(Smad7)、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)蛋白和mRNA水平.采用t检验和方差分析.结果乳腺癌细胞MCF-7中BTG3蛋白(0.18±0.06)和mRNA水平(0.56±0.07)均低于正常乳腺上皮细胞HBL-100 (0.44 ±0.09、1.00 ±0.04)(t=5.375、12.203,P<0.05).与Blank组和NC组比较,Ex-BTG3组MCF-7细胞吸光度(A)值降低(t=4.214、4.185、3.941、3.952,P<0.05),侵袭细胞数降低(t=5.624、5.315,P<O.05),划痕面积升高(t=7.426、7.437,P<0.05),TGF-β1、Vimentin蛋白水平降低(t=3.012、3.024、3.105、3.108,P<0.05),BTG3、Smad7、E-cadherin蛋白水平升高(t=4.516、4.518、3.231、3.118、3.402、3.411,P<0.05).结论乳腺癌组织中BTG3水平下降,过表达BTG3可通过抑制TGF-β1/Smad信号通路抑制乳腺癌细胞增殖和侵袭能力.Objective To investigate the expression of B cell translocation gene 3 (BTG3) in breast cancer tissues and its effect on proliferation and invasion of breast cancer cells. Methods The breast cancer tissue and adjacent cancer tissues from 80 breast cancer patients were selected.The sestern blot and reverse transcription-polymerase chain reaction (RT-PCR) were used to measure the BTG3 protein and mRNA levels in breast cancer tissues and adjacent tissues.The MCF-7 cells were divided into Blank group (no transfection), NC group (transfected with empty vector) and Ex-BTG3 group (transfected with overexpressed BTG3 vector). The cell proliferation was detected by 3-(4, 5-dimethylthiazole-2)-2, 5-diphenyltetrazolium bromide (MTT) assay.The Transwell chamber was used to measure the cell invasiveness ability.The scratch test was used to determine the cell migration ability.The Western blotting and RT-PCR were used to determine the levels of BTG3, transforming growth factor β1 (TGF-β1), human signal transduction molecule 7 (Smad7), E-cadherin, vimentin protein and mRNA. T test and analysis of variance were used. Results The BTG3 protein (0.18±0.06) and mRNA level (0.56±0.07) in breast cancer cells MCF-7 were lower than those in normal breast epithelial cells HBL-100 (0.44±0.09, 1.00±0.04)(t=5.375, 12.203, P<0.05). Compared with the Blank group and the NC group, the optical density value (A) value of MCF-7 cells in the Ex-BTG3 group was decreased (t=4.214, 4.185, 3.941, 3.952, P<0.05), the number of invasive cells was decreased (P<0.05), the scratch area was increased (t=7.426, 7.437, P<0.05), TGF-β1, the level of TGF-β1, Vimentin protein and mRNA were decreased (t=3.012, 3.024, 3.105, 3.108, P<0.05), and the levels of BTG3, Smad7 and E-cadherin protein and mRNA were increased (t=4.516, 4.518, 3.231, 3.118, 3.402, 3.411, P<0.05). Conclusion The level of BTG3 in breast cancer tissues is decreased, and overexpression of BTG3 can inhibit the proliferation and invasion of breast cancer cells by inhibiting TGF-β1/Smad si

关 键 词：B细胞转位基因3 乳腺癌 增殖 侵袭 转化生长因子-β1 人信号转导分子7 上皮-间充质转化 

分 类 号：R737.9[医药卫生—肿瘤]