同源异型框基因翻译基因间RNA在肠癌组织的表达及其生物学功能  

Expression and biological function of long chain non coding RNA-HOX transcript antisense RNA in colorectal cancer

作　　者：赵国刚 运涛 蒋丽丽 白雪巍[3] Zhao Guogang;Yun Tao;Jiang Lili;Bai Xuewei(Department of General Surgery, Tianjin 5th Central Hospital (Peking University Binhai Hospital), Tianjin 300450, China;Department of Radiology, Tianjin 5th Central Hospital (Peking University Binhai Hospital), Tianjin 300450, China;Department of General Surgery, 1st Affiliated Hospital of Harbin Medical University, Harbin 150081, China)

机构地区：[1]天津市第五中心医院(北京大学滨海医院)普外科,300450 [2]天津市第五中心医院(北京大学滨海医院)放射科,300450 [3]哈尔滨医科大学附属第一医院普外科,150081

出　　处：《中华实验外科杂志》2019年第8期1469-1471,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨同源异型框基因翻译基因间RNA (LincRNA-HOTAIR)在人肠癌组织的表达及其与细胞增殖、迁移能力表型的关系.方法采用实时荧光定量聚合酶链反应(Real-time PCR)检测41例肠癌患者的癌组织、癌旁组织和肠癌细胞株LoVo中HOTAIR的相对表达;合成针对HOTAIR的小干扰RNA转染并下调LoVo细胞中HOTAIR的表达水平.采用噻唑蓝(MTF)实验、划痕实验和Transwll实验评价HOTAIR下调前后LoVo细胞增殖和迁移能力变化.应用SPSS 17.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,两组之间比较采用配对t检验.结果肠癌患者癌组织中HOTAIR的相对表达量显著高于癌旁组织(7.96 ±4.15比4.47±2.70),且差异有统计学意义(t=4.520,P<0.01);MTT实验显示,下调HOTAIR表达后,LoVo细胞增殖能力无明显改变(t=1.080,P>0.05),而细胞的迁移能力和穿膜能力明显减低(t=2.540,P<0.05).结论同源异型框基因翻译基因间RNA在人肠癌组织呈现高表达,并与肠癌细胞LoVo的迁移能力有关.Objective To evaluate the long intervening noncoding RNA (LincRNA)-HOX transcript antisense RNA (HOTAIR) expression in colorectal cancer and its biology function of proliferation and metastasis in human colorectal cancer cell line LoVo. Methods Real-time quantitative polymerase chain reaction (Real-time PCR) was applied to analysis the expression of HOTARI in paired colorectal cancer tissues and para-cancer normal colorectal tissues. And the HOTAIR was knocked down by small interfering RNA (siRNA) in LoVo cells. Methyl thiazol tetrazolium (MTT), wound healing and transwell assay were applied to evaluated the cell proliferation and invasion ability. Results HOTAIR expression were 7.96±4.15 and 4.47±2.70 for cancer tissue and para-cancer normal colorectal tissue respectively with statistical differenc (t=4.520, P<0.01);Knocking down HOTAIR in LoVo cells can decrease cell migration/invasion (t=2.540, P<0.05). However, it had no effects on cell proliferation (t=1.080, P>0.05). Conclusion HOTAIR was high expressed in human colorectal cancer tissue and down-regulation of HOTAIR in colorectal cancer can promote cell migration/invasion.

关 键 词：同源异型框基因翻译基因间RNA 肠癌 增殖 侵袭 迁移 

分 类 号：R735.3[医药卫生—肿瘤]