Anti-inflammatory Effect of Total Saponin Fraction from Dioscorea nipponica Makino on Gouty Arthritis and Its Influence on NALP3 Inflammasome  

作　　者：ZHOU Qi YU Dong-hua ZHANG Ning LIU Shu-min 

机构地区：[1]Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China [2]Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang Province, Heilongjiang University of Chinese Medicine, Harbin 150040, China

出　　处：《Chinese Journal of Integrative Medicine》2019年第9期663-670,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.81173618,81403156);China Postdoctoral Science Foundation(No.2015M57036);Heilongjiang Postdoctoral Special Assisted Fund(Nos.LBH-TZ0520,LBH-Z13191);Scientific Fund of Heilongjiang University of Chinese Medicine(No.2013bs05);the Outstanding Innovative Talent Support Programs of Heilongjiang University of Chinese Medicine

摘　　要：Objective: To investigate the mechanism of Chinese herbal medicine Dioscorea nipponica for the treatment of monosodium urate crystals-induced gouty arthritis(GA) in rats. Methods: Sixty male Wistar rats were divided into 6 groups: normal, model, indomethacin and three total saponin(900, 300 and 100 mg/kg) groups. The liver, kidney and serum levels of lysosomal enzymes, antioxidant capacities, and inflammatory factors were measured. In addition, the m RNA and protein levels of the NALP3 inflammasome components in the mononuclear cells of rats’ peripheral blood were analyzed using real-time polymerase chain reaction and Western blotting methods, respectively. Results: Total saponins groups could reduce the activities of β-galactosidase,β-N acetyl glucosamine enzyme,β-glucuronidase, acid phosphatase, and malonaldehyde as wel as the contents of TNF-α, IL-1β and IL-8(all P<0.05). They could also increase the activities of glutathione peroxidase and total superoxide dismutase(both P<0.05). Further studies showed that total saponins groups of high, middle and low doses could all increase the m RNA and protein levels of caspase-1, adapter apoptosis-associated speck-like protein containing a caspase-recruitment domain(ASC) and NALP3 in the mononuclear cells of peripheral blood(all P<0.05). Conclusion: Dioscorea nipponica may treat GA by regulating lysosomal enzymes, antioxidant capacities and the NALP3 inflammasome.Objective: To investigate the mechanism of Chinese herbal medicine Dioscorea nipponica for the treatment of monosodium urate crystals-induced gouty arthritis(GA) in rats. Methods: Sixty male Wistar rats were divided into 6 groups: normal, model, indomethacin and three total saponin(900, 300 and 100 mg/kg) groups. The liver, kidney and serum levels of lysosomal enzymes, antioxidant capacities, and inflammatory factors were measured. In addition, the m RNA and protein levels of the NALP3 inflammasome components in the mononuclear cells of rats’ peripheral blood were analyzed using real-time polymerase chain reaction and Western blotting methods, respectively. Results: Total saponins groups could reduce the activities of β-galactosidase, β-N acetyl glucosamine enzyme, β-glucuronidase, acid phosphatase, and malonaldehyde as wel as the contents of TNF-α, IL-1β and IL-8(all P<0.05). They could also increase the activities of glutathione peroxidase and total superoxide dismutase(both P<0.05). Further studies showed that total saponins groups of high, middle and low doses could all increase the m RNA and protein levels of caspase-1, adapter apoptosis-associated speck-like protein containing a caspase-recruitment domain(ASC) and NALP3 in the mononuclear cells of peripheral blood(all P<0.05). Conclusion: Dioscorea nipponica may treat GA by regulating lysosomal enzymes, antioxidant capacities and the NALP3 inflammasome.

关 键 词：apoptosis-associated speck-like protein containing a caspase-recruitment domain caspase-1 Chinese medicine DIOSCOREA nipponica NALP3 

分 类 号：R[医药卫生]