焦虑状态下去甲肾上腺素激活Akt信号通路对乳腺癌作用研究  

Study on norepinephrine activating Akt signaling pathway in breast cancer under anxiety state

作　　者：欧阳雪岩 朱朕 杨超 王丽 姜峰 丁罡[1,4] OUYANG Xue-yan;ZHU Zhen;YANG Chao;WANG Li;JIANG Feng;DING Gang(Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200092;School of Life Sciences,Shanghai University,Shanghai 200444;Translational Institute for Cancer Pain,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Chongming Branch,Shanghai 202150;Shanghai International Medical Center,Shanghai 200120,China)

机构地区：[1]上海交通大学医学院附属新华医院,上海200092 [2]上海大学生命科学学院,上海200444 [3]上海交通大学医学院附属新华医院崇明分院癌痛转化研究所,上海202150 [4]上海国际医学中心,200120

出　　处：《蚌埠医学院学报》2019年第7期841-845,共5页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81572859);上海市卫生健康委员会资助项目(20184Y0016,201640201,201840003,201740027);上海市崇明区科学技术委员会资助项目(CKY2018-03)

摘　　要：目的:探讨去甲肾上腺素在焦虑状态下对乳腺癌的作用及机制。方法:将20只C57BL/6小鼠分为对照组及模型组,通过接种肿瘤细胞和实验刺激建立焦虑荷瘤小鼠模型,造模结束后检测血清中去甲肾上腺素水平并测量肿瘤体积。人乳腺癌细胞系MDA-MB-231细胞在去甲肾上腺素处理后检测其增殖、迁移、侵袭、细胞周期分布及Akt信号通路的激活状态。结果:模型组小鼠肿瘤体积大于对照组(P<0.05),血清中去甲肾上腺素含量明显升高(P<0.01),且同焦虑程度呈现相关关系(r=0.78)。去甲肾上腺素提高了MDA-MB-231细胞的增殖、迁移和侵袭能力,并且促进DNA复制以及Akt信号通路的激活。结论:去甲肾上腺素在焦虑状态下激活AKt信号通路,促进乳腺癌的发展。Objective: To explore the effects and mechanism of norepinephrine in breast cancer under anxiety state. Methods: Twenty C57BL/6 mice were divided into the control group and model group.The mice models with anxiety tumor-bearing were established using inoculation of tumor cells and experimental stimulation.The serum level of norepinephrine was detected,and the tumor volume was measured after modeling.The proliferation,migration,invasion,cell cycle distribution and activation of Akt signaling pathway of MDA-MB-231 breast cancer cells treated with norepinephrine were detected. Results: The tumor volume in model group was significantly larger than that in control group( P <0.05),and the serum concentration of norepinephrine significantly increased( P <0.01),which was correlated with the anxiety degree( r =0.78).Norepinephrine enhanced the proliferation,migration and invasion of MDA-MB-231 cells,promoted DNA replication,and activated the Akt signaling pathway. Conclusions: Norepinephrine activates the Akt signaling pathway to promote the development of breast cancer under state anxiety.

关 键 词：乳腺肿瘤 焦虑 去甲肾上腺素 AKT信号通路 

分 类 号：R737.9[医药卫生—肿瘤]