拟南芥VHA-c1基因对非生物胁迫的响应  

The Responses of VHA-c1 Gene in Arabidopsis thaliana to Abiotic Stresses

作　　者：韩晓东 郭荣起 高阳[1] 于秀敏 苏杰[4] 张子义[1] 李国婧[1] 王瑞刚[1] HAN Xiaodong;GUO Rongqi;GAO Yang;YU Xiumin;SU Jie;ZHANG Ziyi;LI Guojing;WANG Ruigang(Inner Mongolia Agricultural University,Inner Mongolia Key Laboratory of Plant Stress Physiology and Molecular Biology,Hohhot010018,China;Agricultural Sciences of Hulunbeier,Zalantun162650,China;Institute of Grassland Research of CAAS,Hohhot010018,China;Vocational and Technical College of Inner Mongolia Agricultural University,Baotou014109,China)

机构地区：[1]内蒙古农业大学内蒙古自治区植物逆境生理与分子生物学重点实验室,内蒙古呼和浩特010018 [2]呼伦贝尔市农业科学研究所,内蒙古扎兰屯162650 [3]中国农业科学院草原研究所,内蒙古呼和浩特010018 [4]内蒙古农业大学职业技术学院,内蒙古包头014109

出　　处：《华北农学报》2019年第4期62-66,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30460018);内蒙古自治区科技创新团队建设计划项目(201503004)

摘　　要：为初步探讨V-ATPasec亚基VHA-c1基因在植物生长发育及信号转导中的作用,通过农杆菌介导,将设计的dsDNA片段整合到拟南芥基因组上,筛选获得了能够特异沉默VHA-c1基因的转基因株系c1-1,并对其进行NaCl、ABA和6%葡萄糖胁迫处理。结果表明:在NaCl处理后,沉默株系c1-1的主根伸长量和种子萌发率都有明显的降低,且在浓度为50,100mmol/LNaCl胁迫处理下,转基因株系c1-1的主根伸长量被显著抑制。在特定浓度的ABA和6%葡萄糖处理后,转基因株系c1-1的主根伸长量和种子萌发率也都有一定程度的抑制。然而,在4,8μmol/LABA处理后,野生株系的主根伸长量被显著抑制,与转基因株系c1-1的主根伸长量被抑制效果相比达到了显著水平。沉默VHA-c1基因后,降低了拟南芥对NaCl的耐受性,影响了其生长发育,然而对ABA和葡糖糖的抑制作用却表现为很不敏感,这可能是因为H+-ATPase在盐胁迫信号通路和ABA信号通路中起着不同的调控功能。The vacuolar type H+-ATPase(VHA)is a multi-subunit endomembrane proton pump and plays a important role in plant adaptation and resistance to abiotic stresses.In order to explore the function of VHA subunit c gene(VHA-c1)in plant growth and signal transduction,the designed double strand DNA fragment was integrated into the genome of Arabidopsis thaliana by Agrobacterium mediation,and then a transgenic line c1-1 was screened to specifically silence the coding gene of VHA-c1 subunit.The results showed that the relative root length and the germination rate of seeds in c1-1 line decreased compared with the wild strain under the stress of NaCl.Especially,under the stress of 50,100 mmol/L NaCl,the relative root length of c1-1 line was suppressed sharply.Under the stress of ABA or glucose,the relative root length of c1-1 line and wild strain was suppressed.However,the relative root length of wild strain decreased more obviously than c1-1 line at 4,8μmol/L ABA treatment.It suggested that the silenced VHA-c1 gene significantly reduced the tolerance of Arabidopsis to NaCl,and was insensitive to ABA and glucose stresses.This might be due to the different regulatory functions of H+-ATPase in salt stress and ABA signaling pathways.

关 键 词：液泡ATP酶 基因干扰技术 非生物胁迫 拟南芥 

分 类 号：Q945.78[生物学—植物学]