封闭群实验用鸽微卫星DNA遗传检测方法的初步建立  

Establishment of a genetic monitoring method for a closed colony of laboratary pigeons based on microsatellite DNA

作　　者：王艺宁 刘新梦 章秀林 何洋 张玮 杜小燕[1] 李长龙[1] 陈振文[1] WANG Yining;LIU Xinmeng;ZHANG Xiulin;HE Yang;ZHANG Wei;DU Xiaoyan;LI Changlong;CHEN Zhenwen(School of Basic Medical Science,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学基础医学院

出　　处：《中国比较医学杂志》2019年第8期17-22,共6页Chinese Journal of Comparative Medicine

基　　金：北京市重点专项课题(D181100000518002);国家自然科学基金(31572348,31572341,31772545);国家重点研发计划(2017YFD0501602)

摘　　要：目的建立封闭群实验用鸽微卫星DNA遗传检测方法。方法通过文献检索和SSR Hunter软件筛选,选取和设计了鸽微卫星位点共61个。利用鸽基因组样本进行PCR扩增及条件优化。利用琼脂糖凝胶电泳结果进行初筛,获得等位基因丰富、扩增条带清晰的20个微卫星位点。通过STR扫描比较分析,选取适用于实验用鸽遗传质量检测的位点共16个并应用于两个封闭群鸽群体。结果利用筛选出的16个微卫星DNA位点,初步建立封闭群实验用鸽的遗传检测方法。结论初步建立了基于微卫星DNA的封闭群实验用鸽遗传检测方法,为封闭群实验用鸽种群遗传质量控制奠定基础。Objective To establish a method for genetic detection of pigeon microsatellite DNA in a closed-group experiment.Methods Sixty-one pigeon microsatellite loci were selected and designed through literature search and SSR(simple sequence repeats)Hunter software screening.PCR amplification and condition optimization were performed using pigeon genome samples.Twenty microsatellite loci with abundant alleles and distinct amplification bands were obtained using agarose gel electrophoresis.Through comparative analysis of STR(short tandem repeat)scanning,16 loci suitable for testing the genetic quality of experimental pigeons were selected and applied to two closed-group pigeon populations.Results Sixteen microsatellite DNA loci were selected to establish the genetic detection method of a closed group of pigeons.Conclusions A genetic detection method based on microsatellite DNA is established for a closed group of pigeons.This method lays a foundation for genetic quality control in a closed-group pigeon population.

关 键 词：实验用鸽 微卫星DNA 封闭群 遗传检测技术 

分 类 号：R-33[医药卫生]