实验用鸡的遗传检测方法的初步建立  

Establishment of a genetic monitoring method for laboratory chickens

作　　者：崔爱缺 宫昳頔 章秀林 何洋 张玮 韩凌霞[2] 陈继兰[3] 李长龙[1] 陈洪岩[2] 陈振文[1] CUI Aique;GONG Yidi;ZHANG Xiulin;HE Yang;ZHANG Wei;HAN Lingxia;CHEN Jilan;LI Changlong;CHEN Hongyan;CHEN Zhenwen(Capital Medical University School of Basic Medical Sciences,Beijing 100069,China;Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150069;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193)

机构地区：[1]首都医科大学基础医学院,北京100069 [2]中国农业科学院哈尔滨兽医研究所,哈尔滨150069 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国比较医学杂志》2019年第8期23-30,共8页Chinese Journal of Comparative Medicine

基　　金：北京市重点专项课题(D181100000518002);国家自然科学基金(31572348,31572341,31772545);黑龙江省自然科学基金重点项目(ZD2016006)

摘　　要：目的建立实验用鸡微卫星DNA遗传检测方法。方法通过文献检索分析初步筛选出72个微卫星位点,利用PCR和STR扫描确定扩增效果好、稳定性强的位点。初步确定适合封闭群和单倍型实验用鸡遗传检测的位点组合,并在3个封闭群实验用鸡和3个单倍型实验用鸡的群体进行应用。结果初步确定适用于封闭群实验用鸡遗传检测的28个微卫星位点组合和适用于单倍型实验用鸡遗传检测的14个微卫星位点组合。结论初步建立了实验用鸡的遗传检测方法。Objective To establish a genetic monitoring method for laboratory chickens.Methods Seventy-two microsatellite loci were chosen from the literature.PCR and short tandem repeat(STR)scanning techniques were used to identify the loci with good amplification effects and stability.The loci suitable for genetic monitoring of closed-colony and haplotype laboratory chickens were preliminarily established.This genetic monitoring method was applied in three closed laboratory chicken colonies and three haplotype laboratory chickens.Results We preliminarily determined 28 microsatellite loci for genetic monitoring of closed-colony laboratory chickens and 14 microsatellite loci for genetic testing of haplotype laboratory chickens.Conclusions A genetic monitoring method for laboratory chickens is preliminary established.

关 键 词：实验用鸡 遗传检测方法 微卫星 封闭群 单倍型 

分 类 号：R-33[医药卫生]