下调PHLDB3对胃癌耐药细胞系SGC7901/ADR药物敏感性的影响  

The effect of PHLDB3 downregulation on drug resistance in gastric cancer cell line SGC7901/ADR

作　　者：刘家铭 张静雯 司望利[1] Liu Jiaming;Zhang Jingwen;Si Wangli(Digestive Department, Xi'an Central Hospital, Shaanxi Xi'an 710003, China;Department of Rheumatology and Immunology, Xi'an No.5 Hospital, Shaanxi Xi'an 710082, China)

机构地区：[1]西安市中心医院消化科,陕西西安710003 [2]西安市第五医院风湿免疫六病区,陕西西安710082

出　　处：《现代肿瘤医学》2019年第17期3010-3014,共5页Journal of Modern Oncology

摘　　要：目的:探讨下调PHLDB3对胃癌耐药细胞系SGC7901/ADR药物敏感性的影响。方法:采用实时定量聚合酶链反应(qRT-PCR)和蛋白免疫印迹技术(Western blot)的方法检测胃癌及癌旁正常组织中PHLDB3的表达(分别是20对和10对);qRT-PCR和Western blot检测PHLDB3在胃癌细胞系SGC7901以及胃癌耐药细胞系SGC7901/ADR和SGC7901/VCR中的表达;MTT法检测SGC7901、SGC7901/ADR及转染PHLDB3 siRNA后SGC7901/ADR的半数抑制浓度(IC50)值;流式细胞仪检测细胞凋亡。结果:qRT-PCR和Western blot结果显示:PHLDB3在胃癌组织中的表达明显高于癌旁正常组织(P<0.000 1);PHLDB3在耐药细胞系SGC7901/ADR和SGC7901/VCR中的表达明显高于其亲本细胞系SGC7901(P<0.000 1和P<0.05)。Western blot结果显示:相对于转染PHLDB3 negative control(NC)组,转染PHLDB3 siRNA组的SGC7901/ADR细胞中PHLDB3的表达降低(P<0.005)。MTT结果显示:SGC7901与SGC7901/ADR的IC50值分别为(1.5±0.1)μg/ml与(5.5±0.2)μg/ml(P<0.000 1);SGC7901/ADR转染PHLDB3 siRNA后对顺铂的IC50值明显下降(P<0.05)。流式细胞术(flow cytometry,FCM)检测凋亡,结果显示:下调PHLDB3的表达后,SGC7901/ADR细胞的凋亡率明显增加(P<0.05)。结论:PHLDB3能够促进胃癌细胞系SGC7901/ADR产生多药耐药。Objective:To investigate the influence of PHLDB3 downregulation on drug resistance in gastric cancer drug-resistant cell line SGC7901/ADR.Methods:The expression of PHLDB3 in gastric cancer and adjacent normal tissues was determined by qRT-PCR(20 pairs) and Western blot(10 pairs).The expression of PHLDB3 in gastric cancer cell lines SGC7901,drug-resistant cell line SGC7901/ADR and SGC7901/VCR was detected by qRT-PCR and Western blot.The influence of PHLDB3 knock-down on the IC50 and cell apoptosis of drug-resistant cell line SGC7901/ADR was evaluated using MTT assay and FCM.Results:qRT-PCR and Western blot showed that PHLDB3 was upregulated in gastric cancer compared with adjacent normal tissues(P<0.000 1),and the expression of PHLDB3 was higher in drug-resistant cell line SGC7901/ADR and SGC7901/VCR than that in SGC7901(P<0.000 1 and P<0.05).Compared to SGC7901/ADR transfected with PHLDB3 NC,SGC7901/ADR transfected with PHLDB3 siRNA showed lower PHLDB3 expression(P<0.005).In addition,MTT assay and FCM showed that the decreased IC50 and increased cell apoptosis rate were observed in drug-resistant cell line SGC7901/ADR with PHLDB3 knock-down(P<0.05).Conclusion:PHLDB3 could contribute to the multidrug resistance of gastric cancer cell line SGC7901/ADR.

关 键 词：PHLDB3 胃癌 多药耐药 

分 类 号：R735.2[医药卫生—肿瘤]