原发胃B细胞淋巴瘤PIK3CA基因扩增检测及其对AKT通路的影响  

Detection of PIK3CA gene amplification in primary gastric B cell lymphoma and its effect on AKT pathway

作　　者：翟林柱[1] 刘泽宇 余榕键 傅思莹[3] 朱红 Zhai Linzhu;Liu Zeyu;Yu Rongjian;Fu Siying;Zhu Hong(Cancer Center, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Guang zhou 510405, China;Department of Traditional Chinese Medicine, Guangdong People's Hospital, Guangdong Guangzhou 510060 , China;Department of Pathology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Guang zhou 510405, China)

机构地区：[1]广州中医药大学第一附属医院肿瘤中心,广东广州510405 [2]广东省人民医院中医科,广东广州510060 [3]广州中医药大学第一附属医院病理科,广东广州510405

出　　处：《现代肿瘤医学》2019年第17期3070-3073,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81873147);广东省医学科研基金(编号:B2014176);广州市科技计划项目(编号:201607010384);广州中医药大学高水平大学面上项目(编号:A1-AFD018171Z11069)

摘　　要：目的:检测30例原发胃B细胞淋巴瘤石蜡包埋标本中PIK3CA扩增及蛋白表达,并观察PI3K/AKT通路中AKT激活状况。方法:实时荧光定量PCR检测PIK3CA基因拷贝数,逆转录PCR检测PIK3CA mRNA拷贝数,免疫组化法检测PIK3CA蛋白表达及p-AKT^T473的表达水平。结果:30例患者中,11例(36.7%)患者出现PIK3CA基因扩增,其中MALT淋巴瘤2例(28.6%),套细胞淋巴瘤4例(57.1%),DLBCL 5例(31.3%)。5例(16.7%)患者出现PIK3CA mRNA基因扩增,8例(26.7%)PIK3CA蛋白阳性表达。PIK3CA基因DNA扩增与PIK3CA蛋白表达之间存在统计学相关性(P=0.007),但与mRNA扩增无关(P=0.865)。PIK3CA蛋白与磷酸化AKT^T473表达之间存在统计学差异(P=0.012)。结论:原发胃B细胞淋巴瘤中存在PIK3CA扩增和蛋白表达,并可能进一步激活下游的AKT分子。Objective:To detect PIK3 CA amplification and protein expression in paraffin embedded specimens of 30 cases with primary gastric B cell lymphoma,and to observe the activation of AKT in PI3 K/Akt pathway.Methods:The copy number of PIK3 CA gene was detected by real-time fluorescence quantitative PCR.The copy number of PIK3 CA mRNA was detected by reverse transcriptase PCR,and the expression of PIK3 CA protein and the expression of p-AKT^T473 were detected by immunohistochemistry.Results:Of the 30 patients,11 patients had PIK3 CA gene amplification(36.7%),including 2 MALT lymphomas(28.6%),4 mantle cell lymphomas(57.1%),and 5 DLBCLs(31.3%).PIK3 CA mRNA amplification was found in 5 patients(16.7%),and 8 cases were positive for PIK3 CA protein(26.7%).There was a statistical correlation between the DNA amplification of PIK3 CA gene and the expression of PIK3 CA(P=0.007),but it was not related to mRNA amplification(P=0.865).There was a significant relation between the expression of PIK3 CA protein and phosphorylated AKTT473(P=0.012).Conclusion:PIK3 CA amplification and protein expression can be observed in primary gastric B cell lymphoma and may further activate downstream AKT molecules.

关 键 词：原发胃B细胞淋巴瘤 PIK3CA 磷酸化AKT 

分 类 号：R733[医药卫生—肿瘤]