上调BRMS1基因抑制IL-6受体表达调控鼻咽癌细胞增殖和转移的机制研究  

Mechanism of BRMS1 gene suppressing IL-6 receptor expression to regulate proliferation and metastasis of nasopharyngeal carcinoma cells

作　　者：邢兵[1] 刘国红 张国顺 

机构地区：[1]河南护理职业学院,安阳455000 [2]安阳市妇幼保健院,安阳455000 [3]安阳地区医院,安阳455000

出　　处：《中国免疫学杂志》2019年第16期1987-1991,1998,共6页Chinese Journal of Immunology

摘　　要：目的:探讨上调乳腺癌转移抑制基因1(BRMS1)抑制IL-6受体对鼻咽癌细胞增殖、转移的影响。方法:将人鼻咽癌SUNE-1细胞分为空白组、pcDNA3.1组和pcDNA3.1-BRMS1组,Western blot检测BRMS1、IL-6、IL-6R、PCNA、E-cadenin、Vimentin、STAT3和p-STAT3的蛋白表达。MTT法及Transwell小室检测细胞活力和迁移能力。小鼠注射pcDNA3.1-BRMS1的细胞悬液,检测4周后肿瘤体积大小及肿瘤组织PCNA、E-cadenin、Vimentin、STAT3和p-STAT3蛋白表达。结果:与空白组比较,pcDNA3.1-BRMS1组鼻咽癌细胞BRMS1和E-cadenin蛋白表达明显升高,IL-6、IL-6R、PCNA、Vimentin和p-STAT3的蛋白表达明显降低,细胞活力明显降低,细胞迁移能力明显降低(P<0.05)。从第3周起,pcDNA3.1-BRMS1组小鼠肿瘤体积与空白组肿瘤体积相比有较大变化,差异有统计学意义(P<0.05)。小鼠肿瘤组织PCNA、E-cadenin、Vimentin和p-STAT3的蛋白表达明显低于空白组,E-cadenin表达高于空白组(P<0.05)。结论:BRMS1可抑制IL-6受体,下调STAT3信号,进而抑制鼻咽癌细胞的增殖和转移能力。Objective:To investigate the effect of up regulation of BRMS1 gene on the proliferation and metastasis of nasopharyngeal carcinoma(NPC) cells by inhibiting IL-6 receptor.Methods: Human nasopharyngeal carcinoma SUNE-1 cells were divided into blank group,pcDNA3.1 group and pcDNA3.1-BRMS1 group.The expressions of BRMS1,IL-6,IL-6 R,PCNA,E-cadenin,Vimentin,STAT3 and p-STAT3 were detected by Western blot.Cell viability and migration ability were detected by MTT assay and Transwell chamber.Mice were injected with pcDNA3.1-BRMS1 cell suspension for 4 weeks,the tumor size and the expression of PCNA,E-cadenin,Vimentin,STAT3 and p-STAT3 in tumor tissues were detected.Results: Compared with the blank group,the expression of BRMS1 and E-cadenin protein was significantly increased,the expression of IL-6,IL-6 R,PCNA,Vimentin and p-STAT3 in NPC cells in pcDNA3.1-BRMS1 group was significantly decreased,the cell viability was significantly decreased,and the cell migration ability was significantly decreased(P<0.05).From the third week,the tumor size in pcDNA3.1-BRMS1 group was significantly different from that in blank group(P<0.05).The expression of PCNA,E-cadenin,Vimentin and p-STAT3 in tumor tissues of mice was significantly lower than that in control group,and E-cadenin was higher than that in control group(P<0.05).Conclusion: Up regulation of BRMS1 can inhibit IL-6 receptor,down regulate STAT3 signal,and then inhibit the proliferation and metastasis of NPC cells.

关 键 词：鼻咽癌 BRMS1基因 IL-6/STAT3信号 增殖 转移 

分 类 号：R736.1[医药卫生—肿瘤]