miRNA-210对人乳腺癌细胞增殖和迁移及侵袭的影响  

Effects of miRNA-210 on the proliferation,migration and invasion of human breast cancer cells

作　　者：黄琦[1] 王芳 田进海[1] 于晶晶[1] 王嘉[1] 王立斌[1] Huang Qi;Wang Fang;Tian Jinhai;Yu Jingjing;Wang Jia;Wang Lib(Ningxia Medical University General Hospital,Yinchuan,Ningxia 750004,China;Ningxia Hui Autonomous Region Aquatic Technology Promotion Station,Yinchuan,Ningxia 750001,China)

机构地区：[1]宁夏医科大学总医院,宁夏银川750004 [2]宁夏回族自治区水产技术推广站,宁夏银川750001

出　　处：《医药前沿》2019年第22期18-19,共2页Journal of Frontiers of Medicine

摘　　要：目的:研究与分析miRNA-210对人乳腺癌细胞增殖、迁移和侵袭的影响。方法:选取来院就诊与治疗的患有乳腺癌疾病的患者20例,选取时间为2016年8月-2018年8月,对患者的乳腺癌组织、癌旁组织、乳腺癌细胞MDA-MB-231以及正常乳腺细胞等组织采取real-time PCR检测,观察miR-210的表达情况.同时将miR-210inhibitor转染至MDA-MB-231细胞中,采取Lipofectamine 2000,对miR-210采用荧光显微镜进行检测,检测转染效率.对细胞采取Transwell法来检测其迁移和侵袭情况.结果:乳腺癌组织、MDA-MB-231细胞与癌旁组织、正常乳腺细胞等组织中的miR-210表达水平差异较大,前者的表达水平显著较高,差异具有统计学意义(P<0.05).miR-210inhibitor转染效率为(88.28±2.99)%,经过转染后,其细胞的增殖能力较转染前显著下降,且细胞的迁移和侵袭能力受到明显抑制.结论:miRNA-210对人乳腺癌细胞增殖、迁移和侵袭的影响较大,可以明显减弱其能力。Objective To study and analyze the effects of miRNA-210 on the proliferation,migration and invasion of human breast cancer cells.Methods 20 patients with breast cancer were selected from August 2016 to August 2018.The expression of miR-210 was observed in breast cancer tissues,pericancerous tissues,breast cancer cells MDA-MB-231 and normal breast cells.At the same time,miR-210 inhibitor was transfected into MDA-MB-231 cells.Lipofectamine 2000 was used to detect miR-210 using a fluorescence microscope to detect the transfection efficiency Transpell method is used to detect the migration and invasion of cells.Results The expression level of miR-210 in breast cancer tissue,MDA-MB-231 cells and other tissues was significantly different from that of normal breast cells.The expression level of the former was significantly higher and statistically significant(P<0.05).The transfection efficiency of miR-210 inhibitor was(88.28±2.99)%.After transfection,the cell's value-added capacity decreased significantly compared with that before transfection,and the cell's migration and invasion ability were significantly inhibited.Conclusion MiRNA-210 has a great influence on the proliferation,migration and invasion ofhuman breast cancer cells,and can obviously weaken its ability.

关 键 词：miRNA-210 人乳腺癌细胞 增殖 迁移 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]