一株高产葡聚糖肠膜状明串珠菌肠膜亚种的筛选及发酵条件优化  

Screening and optimization of fermentation conditions of Leuconostoc mesenteroides subsp. mesenteroides with efficient glucan producing ability

作　　者：孙擎 张恕铭 谭霄 曾林 张庆 SUN Qing;ZHANG Shuming;TAN Xiao;ZENG Lin;ZHANG Qing(Key Laboratory of Food Biotechnology of Sichuan Provincial,College of Food and Bioengineering,Biotechnology Institute of Ancient Brewing,Xihua University,Chengdu 610039;Quality Inspection Co.,Ltd.of Sichuan,Luzhou 646000)

机构地区：[1]西华大学食品与生物工程学院,四川省食品生物技术重点实验室,西华大学古法发酵(酿造)生物技术研究所,成都610039 [2]四川国检检测有限责任公司,泸州646000

出　　处：《食品科技》2019年第7期16-23,共8页Food Science and Technology

基　　金：四川省科技计划项目(2018NFP0111);四川省科技成果转化项目(2016CC0074);四川省教育厅重点项目(18ZA0447);西华大学大健康管理发展中心重点项目(szjj2017-037)

摘　　要：为拓宽高产葡聚糖的种质资源,以四川泡菜为分离源从中筛选高产葡聚糖的乳酸菌菌株,采用苯酚硫酸法对筛选菌株进行葡聚糖产量测定,并对菌株进行生理生化及16S rRNA基因和rpoB看家基因序列的同源性分析鉴定和高产葡聚糖发酵条件优化。结果表明:菌株LM187产葡聚糖能力较强,达到38.24 g/L,进一步鉴定为肠膜状明串珠菌肠膜亚种(Leuconostoc mesenteroidessubsp.mesenteroides);采用响应面中心组合实验优化产葡聚糖发酵条件,得到最优培养条件为发酵时间35 h、发酵温度30℃、蔗糖质量分数21%和接种量3%。在此优化条件下,葡聚糖产量为56.54 g/L,较优化前提高了1.48倍。肠膜状明串珠菌肠膜亚种LM187具有高产葡聚糖的能力,可作为开发保健型泡菜的潜在优质功能性微生物资源。In order to broaden and evaluate the microbial resources with capability of producing glucan,lactic acid bacteria strains were isolated from Sichuan pickles.Determination of glucan was carried out by phenol-sulfuric acid method.The strain was identified using physiological,biochemical tests,and the homology analysis of 16S rRNA gene and rpoB housekeeping gene sequences.And the fermentation conditions were also optimized using the response surface methodology.The results showed that strain LM187 which was identified as Leuconostoc mesenteroides subsp.mesenteroides,has higher ability to produce glucan,up to 38.24 g/L.The optimal fermentation conditions were as follows:fermentation time 35 h,fermentation temperature 30℃,sucrose concentration 21%and inoculum amount 3%.Under the optimized conditions,the production of glucan by strain LM187 reached 56.54 g/L,which was 1.48 fold higher than before optimization.Leuconostoc mesenteroides subsp.mesenteroides LM187,a highly efficient glucan-producing strain,can be used as a potential strain resource for the development of functional Sichuan pickles.

关 键 词：葡聚糖 筛选 肠膜状明串珠菌肠膜亚种 响应面分析法 

分 类 号：TS201.3[轻工技术与工程—食品科学]