circ_0009910在胃癌中的表达及其预后价值研究  

Expression and prognostic values of circ_0009910 in gastric cancer

作　　者：刘铭[1] 张乐[1] 刘凯东[1] 白月奎[1] Liu Ming;Zhang Le;Liu Kaidong;Bai Yuekui(Department of General Surgery, Beijing Haidian Hosiptal, Beijing 100080, China)

机构地区：[1]北京市海淀医院普通外科,100080

出　　处：《国际外科学杂志》2019年第8期530-535,F0003,共7页International Journal of Surgery

摘　　要：目的通过检测分析circ_0009910在胃癌中的表达和意义,评估其与胃癌临床病理特征的相关性和预后价值。方法北京市海淀区医院2010年2月—2014年11月收治胃癌患者129例,术中采集胃癌患者肿瘤组织和癌旁非肿瘤组织各129例,qRT-PCR实验检测circ_0009910的表达水平,分析其与患者临床病理特征的相关性,Kaplan-Meier法作生存分析,COX回归分析circ_0009910的预后价值。结果Circ_0009910在肿瘤组织中的表达水平显著高于癌旁非肿瘤组织(P<0.01)。其表达水平与患者临床分期(P=0.032)、远处转移(P=0.018)及肿瘤分化(P=0.007)显著相关,而与年龄、性别、Lauren分型、肿瘤大小未表现明显相关性(P>0.05)。胃癌较晚期(Ⅲ/Ⅳ期)、远处转移及低分化的患者中,circ_0009910表达水平更高。阳性表达组患者的生存率显著低于阴性表达组(P=0.001 5),5年生存率分别为29.5%和53.2%;circ_0009910表达水平(P=0.006)、远处转移(P=0.009)及肿瘤分化(P=0.035)是患者总生存期的独立风险因子。结论Circ_0009910在胃癌患者肿瘤组织中的表达水平显著高于癌旁非肿瘤组织,并与临床较晚期(Ⅲ/Ⅳ期)、远处转移、肿瘤低分化相关。Circ_0009910在肿瘤组织中表达水平较高的患者,生存率较低,是生存期的独立风险因子。Objective To study the expression and significance of circ_0009910 in gastric cancer, and evaluate its correlation with clinicopathological characteristics and prognostic value. Methods From Feb. 2010 to Nov. 2014, there were 129 gastric cancer patients in Beijing Haidian Hospital. One hundred and twenty-nine cases of tumor tissues and 129 paracancerous non-tumor tissues were collected from gastric cancer patients, intraoperatively. The expression of circ_0009910 was detected by qRT-PCR experiment. Then the correlations of circ_0009910 with major clinicopathological characteristics were analyzed. The Kaplan-Meier method was used to analyze overall survival of patients. A Cox proportional hazards regression analysis was used for multivariate analyses of prognostic values. Results The expression level of circ_0009910 in tumor tissues of patients was significantly higher than that in paracancerous non-tumor tissues (P<0.01). The expression level of circ_0009910 in patients was significantly associated with clinical stage (P=0.032), distant metastasis (P=0.018) and differentiation (P=0.007), respectively. There was no obvious correlation of the expression level of circ_0009910 with age, gender, Lauren′s classification, tumor diameter (P>0.05). And the expression level of circ_0009910 was higher in patients with advanced cancer (stage Ⅲ/Ⅳ), distant metastasis and poor differentiation. Survival rate of patients in positive group was significantly lower than negative group (P=0.001 5), with 5-year survival rate were about 29.5% and 53.2%, respectively. circ_0009910 (P=0.006), distant metastasis (P=0.009) and differentiation (P=0.035) were independent risk factors of overall survival of patients, respectively. Conclusions The expression level of circ_0009910 in tumor tissues of gastric cancer patients was significantly higher than that in paracancerous non-tumor tissues, which was significantly correlated with clinical stage, distant metastasis and differentiation. Survival rate was lower in patients with higher ex

关 键 词：胃肿瘤 预后 组织 循环RNA circ_0009910 

分 类 号：R735.2[医药卫生—肿瘤]