Determination of iohexol by capillary blood microsampling and UHPLC-MS/MS  

作　　者：Valentin Ion Caroline Legoff Etienne Cavalier Pierre Delanaye Anne-Catherine Servais Daniela-Lucia Muntean Marianne Fillet 

机构地区：[1]Laboratory for the Analysis of Medicines (LAM), Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, Liege, Belgium [2]Analytical Chemistry and Drug Analysis Department, Faculty of Pharmacy, University of Medicine Pharmacy Science and Technology from Tirgu Mures, 540139, Tirgu Mures, Romania [3]Department of Clinical Chemistry, Center for Interdisciplinary Research on Medicines (CIRM), University of Liege, CHU Sart-Tilman, Liege, Belgium [4]Department of Nephrology-Dialysis-Transplantation, University of Liege, CHU Sart-Tilman, Liege, Belgium

出　　处：《Journal of Pharmaceutical Analysis》2019年第4期259-265,共7页药物分析学报（英文版）

摘　　要：One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN■ device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 mL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 mg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate(GFR).Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance,being neither secreted nor reabsorbed at the tubular level.This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood,prelevated using volumetric absorbative microsampling(VAMS)systems.As an alternative to VAMS,a brand new HemaPEN~?device for micro-prelevation was also tested.A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed.The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days.By collecting only 10 μL of blood,iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250μg/mL Due to the analyte stability in VAMS for up to 12 days,this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.

关 键 词：MICROSAMPLING IOHEXOL VAMS LC-MS/MS HemaPEN 

分 类 号：R[医药卫生]