甜叶菊EST序列特征分析与分子标记开发  

Feature Analysis and Molecular Marker Development of EST-SSR Sequence in Stevia rebaudiana

作　　者：盛文涛[1] 邓建兰 饶友生[1] 柴学文[1] 邹鑫 ShengWentao;Deng Jianlan;Rao Yousheng;Chai Xuewen;Zou Xin(Department of Biological Technology,Nanchang Normal University,Nanchang,330032)

机构地区：[1]南昌师范学院生物技术研究所

出　　处：《分子植物育种》2019年第15期5045-5050,共6页Molecular Plant Breeding

基　　金：江西省教育厅科技项目(GJJ161243);南昌师范学院11531工程项目共同资助

摘　　要：为了明确甜叶菊EST序列中SSR的总体特点,开发甜叶菊EST-SSR分子标记,为利用EST-SSR标记开展甜叶菊分子遗传研究提供科学依据。本研究从NCBI数据库中下载获得了5 646条EST序列,利用在线软件SSRtool和Websat检测所含SSR位点进行分析。结果共检索出88个SSR位点序列,分布于79条EST序列中,检出率为1.6%。其中,六核苷酸重复基元的EST-SSR占主导地位,占总SSR数目的 63.7%。利用在线软件Primer 3.0设计了62对EST-SSR引物,经筛选验证获得了多态性较好的引物32对,表明通过甜叶菊EST序列开发SSR标记是可行的。In order to clarify the overall characteristics of SSR in EST sequence, EST-SSR molecular marker was developed to provide the scientific basis for the molecular genetic research of Stevia rebaudiana by using EST-SSR marker. 5 646 ESTs sequences were downloaded from the NCBI database, the online software of SSRtool and Websat were used to detect and analyze the SSR loci. A total of 88 SSR loci sequences were retrieved, which were distributed in 79 EST sequences, and the detection rate was 1.6%. Among them, the repeat motif of hexanucleotide was the main type in EST-SSR sequences, accounting for 63.7% of the total SSR number. 62 pairs of EST-SSR primers were designed by using the online software Primer 3.0, 32 pairs of primers with good polymorphism were obtained after screening and verification, indicating that it is feasible to develop SSR marker through EST sequences in Stevia rebaudiana.

关 键 词：甜叶菊 NCBI 多态性 EST-SSR 

分 类 号：S566.9[农业科学—作物学]