沙利度胺对膜性肾病及VEGF、PLA2R、TNF-α等因子的影响  

Effects of thalidomide on membranous nephropathy and factors such as VEGF,PLA2R and TNF-α

作　　者：高宏宇 王晔[1] 张莉 张国英[1] 刘秋菊[2] 陈超[1] GAO Hong-yu;WANG Ye;ZHANG Li;ZHANG Guo-ying;LIU Qiu-ju;CHEN Chao(Department of Nephrology,the NO.2 Hospital of Baoding,Hebei Baoding 071000,China;Department of Nephrology,Affiliated Hospital of Hebei University,Hebei Baoding 071030,China)

机构地区：[1]保定市第二医院肾内科,河北保定071000 [2]河北大学附属医院肾内科,河北保定071030

出　　处：《临床药物治疗杂志》2019年第7期70-73,88,共5页Clinical Medication Journal

基　　金：河北省保定市科技计划项目(18ZF013)

摘　　要：目的探讨沙利度胺对膜性肾病(membranous nephropathy,MN)小鼠肾脏病理及血管内皮生长因子(vascular endothelial growth factor,VEGF)、PLA2R、TNF-α等因子的影响。方法选择雄性小鼠60只,随机分为正常组(20只)、模型组(20只)和沙利度胺组(20只),建立MN小鼠模型,观察小鼠肾脏形态学、血清中TNF-α变化情况、肾组织VEGF、PLA2R表达情况。结果实验第8周时,正常组肾组织未见病理改变,模型组肾小球增大,系膜细胞及基质增生,沙利度胺组肾组织病理改变较模型组有不同程度的改善。实验第8周时,正常组PLA2R及VEGF表达明显低于其他2组,沙利度胺组PLA2R及VEGF表达低于模型组,正常组血清中TNF-α水平低于其他2组,沙利度胺组TNF-α水平低于模型组(P<0.05)。结论沙利度胺可下调MN小鼠肾组织VEGF、PLA2R的表达,对其肾脏有保护作用,并可减少血液中TNF-α量,达到缓解MN病情进展的效果。Objective To investigate the effects of thalidomide on renal pathology and factors such as vascular endothelial growth factor(VEGF),PLA2R and TNF-α in mice with membranous nephropathy(MN). Methods Sixty male mice were randomly divided into normal group(20 mice),model group(20 mice),and thalidomide group(20 mice). The MN mouse model was established,and the morphology of kidney,the change of TNF-α in serum,the expression of VEGF and PLA2R in kidney tissue were observed. Results At the 8th week of the experiment,no pathological changes were found in the normal group;glomerulus enlargement,mesangial cells and matrix proliferation in the model group;and the pathological changes of renal tissue in the thalidomide group were improved compared with the model group.At the 8th week of the experiment,the expression of PLA2R and VEGF in the normal group was significantly lower than that in the other 2 groups.The expression of PLA2R and VEGF in the thalidomide group was lower than that in the model group.The level of TNF-α in the normal group was lower than that in the other 2 groups,and the level of TNF-α in the thalidomide group was lower than that in the model group(P<0.05). Conclusion Thalidomide can down-regulate the expression of VEGF and PLA2R in kidney tissue of MN mice,protect their kidneys,and reduce the levels of TNF-α in blood,so as to alleviate the progression of MN.

关 键 词：沙利度胺 膜性肾病 VEGF PLA2R TNF-Α 

分 类 号：R741[医药卫生—神经病学与精神病学]