间歇投喂及越冬对草鱼幼鱼肝脏抗氧化能力的影响  

Effects of Intermittent Feeding and Overwintering on the Antioxidant Defenses of Juvenile Grass Carp (Ctenopharyngodon idellus)

作　　者：钟娟 沐璟玮 李鹏程 姚峰[1] 岳鼎鼎 陈路 杨严鸥 Zhong Juan;Mu Jingwei;Li Pengcheng;Yao Feng;Yue Dingding;Chen Lu;Yang Yanou(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,Anhui;College of Life Science,Anhui Normal University,Wuhu 241000, Anhui;Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture and Rural Affairs,Guangdong Evergreen Feed Industry Co. Ltd.,Zhanjiang 524094,Guangdong)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽师范大学生命科学学院,安徽芜湖241000 [3]农业农村部华南水产与畜禽饲料重点实验室,广东恒兴饲料实业股份有限公司,广东湛江524094

出　　处：《长江大学学报（自然科学版）》2019年第8期88-91,I0006,共5页Journal of Yangtze University(Natural Science Edition)

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07103-004);安徽省自然科学基金项目(1808085MC63)

摘　　要：在25℃水温、自然光照下,设定4种间歇性饥饿投喂方式(连续投喂S 0(对照组),投喂6d后饥饿1d(S 1/6 ),投喂5d后饥饿2d(S 2/5 ),投喂4d后饥饿3d(S 3/4 ))饲养草鱼( Ctenopharyngodon idellus )幼鱼,养殖8周后停止喂食,测定试验鱼生长及肝脏抗氧化指标;再将水温逐渐下降到自然水温(4~9℃),经过56d越冬后,再测定肝脏抗氧化指标。结果显示,S 1/6 与S 2/5 组养殖结束时终末湿重及干重均与对照组无显著差异( P > 0.05),S 3/4 组终末湿重及干重则显著低于对照组( P < 0.05);在越冬前后,不同饥饿处理对超氧化物歧化酶活性均没有显著影响( P > 0.05),过氧化氢酶活性越冬前随着饥饿时间增加显著下降,越冬后则是显著上升( P < 0.05),但同一试验组则是越冬后显著低于越冬前;丙二醛与维生素E含量变化趋势一致,越冬前两者含量随着饥饿时间增加均显著上升后再下降,越冬后则是显著下降后再上升( P < 0.05);谷胱甘肽过氧化物酶活性在越冬随着饥饿时间增加均显著上升后再下降,越冬后则是显著上升( P < 0.05)。研究结果表明,适当的间歇饥饿不会影响草鱼的体重增长,但无论何种间歇饥饿都会导致草鱼肝脏的抗氧化能力下降;经历越冬后,与连续投喂的对照组相比,3个间歇投喂组仍然保持抗氧化应激状态。In order to investigate the antioxidant defenses of grass carp(Ctenopharyngodon idellus)in different feeding regimes,a 56d growth trial is conducted in four feeding regimes at(25±1)℃and in natural light.In the first regime,fishes are fed to satiation during the whole experimental period,and in the second,third and fourth regime,fishes are fed to satiation for 6 days,5 days and 4 days respectively,and then are deprived of food for 1 day,2 days and 3 days respectively.The four regimes are repeated and regarded as S0(control group,CP),S1/6,S2/5 and S3/4.Next,the fishes go into 56d over-wintering period at 4~9℃.The results show that:for S1/6and S2/5 groups,both the final body wet weights and dry matter weights catch up with CP(P>0.05),and for S3/4 group,the final wet and dry weights are lower significantly compared with CP(P<0.05);superoxide dismutase(SOD)activities are not affected significantly by feeding modes before or after overwintering.Catalase(CAT)activity decreases before overwintering,and increases significantly with the increasing of starvation time after overwintering(P<0.05),and for each group the CAT activity decreases significantly after overwintering than before overwintering(P<0.05).The contents of malondialdehyde(MDA)and vitamin E(VE)increase firstly and then decrease significantly with the increasing of starvation time before overwintering(P<0.05),and both of them decrease firstly and then increases significantly after overwintering(P<0.05).Glutathione peroxidase activities increase firstly and then decrease significantly with the increasing of starvation time before overwintering(P<0.05),and increase significantly with the increasing of starvation time after overwintering(P<0.05).The results indicate that some intermittent starvation would not affect the final body weights,while each intermittent starvation leads to lower liver antioxidant ability compared with CP,and three experimental groups still maintain antioxidant stress states after overwintering.

关 键 词：草鱼( CTENOPHARYNGODON IDELLUS ) 饥饿 越冬 生化成分 抗氧化 

分 类 号：S965.112[农业科学—水产养殖]