MCP-1中和抗体鞘内注射对骨癌痛吗啡耐受大鼠OX-42和炎症因子表达的作用  

Effects of intrathecal injection of MCP-1 neutralizing antibody on expression of OX-42 and inflammatory factor in morphineresistant rats with bone cancer pain

作　　者：丰磊 白芬芬 赵晓媛[2] 刘献文[1] 刘磊[1] FENG Lei;BAI Fenfen;ZHAO Xiaoyuan;LIU Xianwen;LIU Lei(Department of Anesthesiology, Liaocheng People’s Hospital, Liaocheng 252000,China;Zaozhuang Vocational College, Zaozhuang 277800, China)

机构地区：[1]山东省聊城市人民医院麻醉科,山东聊城252000 [2]山东省枣庄职业学院,山东枣庄277800

出　　处：《中国医药科学》2019年第15期27-30,37,共5页China Medicine And Pharmacy

基　　金：山东省自然科学基金联合专项（ZR2014HL036）;山东省医药卫生科技发展计划项目（2017WS507）

摘　　要：目的研究MCP-1中和抗体鞘内注射对骨癌痛吗啡耐受大鼠脊髓OX-42和炎症因子TNF-α、IL-1、IL-6表达的作用。方法应用Walker256肿瘤细胞胫骨骨髓腔注射和鞘内注射吗啡建立大鼠骨癌痛吗啡耐受动物模型,采用免疫组化、ELISA检测方法观察MCP-1中和抗体鞘内注射对骨癌痛吗啡耐受大鼠脊髓OX-42和炎症因子TNF-α、IL-1、IL-6表达的作用。结果BM+Ab组大鼠MCP-1中和抗体鞘内注射后脊髓OX-42和炎症因子TNF-α、IL-1、IL-6表达显著减少。结论MCP-1可能通过激活小胶质细胞(OX-42)引发炎症反应从而导致骨癌痛吗啡耐受。Objective To study the effect of intrathecal injection of MCP-1 neutralizing antibody on expressions of spinal cord OX-42 and inflammatory factors such as TNF-α, IL-1 and IL-6 in morphine-resistant rats with bone cancer pain. Methods Animal models of morphine-resistant rats with bone cancer pain were established by tibial intramedullary injection and intramuscular injection of Walker 256 tumor cells. Immunohistochemistry and ELISA were used to observe the effects of intrathecal injection of MCP-1 neutralizing antibody on expressions of spinal cord OX-42 and inflammatory factors such as TNF-α, IL-1 and IL-6 in morphine-resistant rats with bone cancer pain. Results After intrathecal injection of MCP-1 neutralizing antibody, expressions of spinal cord OX-42 and inflammatory factors such as TNF-α, IL-1 and IL-6 in BM+Ab group were significantly reduced. Conclusion MCP-1 may cause inflammatory response by activating microglia (OX-42) leading to bone cancer pain and morphine tolerance.

关 键 词：癌痛 吗啡耐受 OX-42 炎性细胞因子 

分 类 号：R738.1[医药卫生—肿瘤]