多巴胺D2受体和5-HT1B受体在RLS大鼠模型PAG中的共定位  

The co-localization of dopamine D2 receptor and 5-HT1B receptor in PAG of RLS rat model

作　　者：陈强[1] 章正祥[1] 戚观树[1] 周会霞 邵敏峰 黄春华[4] Chen Qiang

机构地区：[1]浙江中医药大学附属第一医院(浙江省中医院),310006 [2]杭州市老年病医院,310022 [3]杭州市余杭区第一人民医院,311100 [4]江西中医药大学附属医院,330006

出　　处：《浙江临床医学》2019年第8期1021-1022,1025,共3页Zhejiang Clinical Medical Journal

基　　金：国家自然科学基金资助项目(81302938);浙江省自然科学基金(LY18H270009);浙江省中医药优秀青年人才基金计划(2013ZQ015);浙江省中医药科学研究基金项目(2017ZA047);浙江省医药卫生科研基金项目(2017KY118,2017KY509);2018年浙江省医药卫生科研面上项目(2018KY549).

摘　　要：目的探索多巴胺D2受体与5HT1B受体是否共同参与不安腿综合征(RLS)的调控.方法建立RLS大鼠模型,取脑组织对中脑导水管周围灰质(PAG)部位进行切片,用免疫荧光双染检测多巴胺D2受体、5-HT1B受体.结果荧光染色显示多巴胺D2受体、5-HT1B受体共存于PAG同一细胞上.结论 PAG共同表达多巴胺D2受体与5HT1B受体,两种受体可能共同参与了RLS的调控.Objective To explore whether dopamine D2 receptor and 5HT1B receptor are involved in the regulation of RLS. Methods The rat model of RLS was established,and the PAG site was sliced after taking brain tissue. The dopamine D2 receptor and 5-HT1B receptor were detected by immunofluorescence double staining. Results Fluorescent staining showed that the dopamine D2 receptor and 5-HT1B receptor coexisted on the same cell. Conclusion PAG co-expresses dopamine D2 receptor and 5HT1B receptor,and both receptors may participate in the regulation of RLS.

关 键 词：多巴胺D2受体 5-HT1B受体 RLS A11 PAG 

分 类 号：R742.5[医药卫生—神经病学与精神病学]