miR-135a-5p和TXNIP在甲状腺乳头状癌组织中的表达及意义  

Expressions and significances of miR-135a-5p and TXNIP in papillary thyroid cancer

作　　者：张莉 朱蕾 白洁[2] Zhang Li;Zhu Lei;Bai Jie(Department of Endocrinology,Shandong Provincial Third Hospital,Jinan 250013,China;Department of Endocrinology,Zhengzhou Third Hospital,Zhengzhou 450000,China)

机构地区：[1]山东省立第三医院内分泌科,济南250013 [2]郑州市第三人民医院内分泌科,450000

出　　处：《中国医师杂志》2019年第8期1195-1198,1204,共5页Journal of Chinese Physician

摘　　要：目的检测甲状腺乳头状癌(PTC)肿瘤组织miR-135a-5p及硫氧还蛋白相互作用蛋白(TXNIP)的表达,并分析其临床意义。方法选取2017年3月至2018年3月在本院手术且经病理证实为PTC患者48例,收集患者肿瘤组织及癌旁组织,利用SP法和Western blot法检测TXNIP蛋白表达情况,实时定量PCR(qRT-PCR)法检测miR-135a-5p表达情况。结果 PTC患者癌旁组织中TXNIP蛋白阳性表达率显著高于肿瘤组织(P<0.05);PTC患者肿瘤组织中TXNIP蛋白表达水平、miR-135a-5p表达水平显著低于癌旁组织(P<0.05);PTC肿瘤直径≥2 cm、淋巴结转移的患者中,miR-135a-5p低表达的患者比例显著高于高表达者(P<0.05);PTC肿瘤直径≥2 cm、淋巴结转移的患者中,TXNIP蛋白阴性表达的患者比例显著高于阳性表达患者(P<0.05);PTC患者肿瘤组织miR-135a-5p与TXNIP蛋白表达水平呈正相关(P<0.05)。结论 PTC患者肿瘤组织中miR-135a-5p、TXNIP蛋白表达水平低于癌旁组织,二者表达水平正相关,与肿瘤直径增加、淋巴结转移有关。miR-135a-5p、TXNIP蛋白低表达可能与PTC肿瘤发生及恶化有关。Objective To detect the expression of miR-135a-5p and thioredoxin interaction protein (TXNIP) in papillary thyroid carcinoma (PTC) and to analyze their clinical significances.Methods 48 patients with PTC who underwent operation and confirmed by pathological examination in Shandong Provincial Third Hospital from March 2017 to March 2018 were selected.Tumor tissues and adjacent tissues were collected.The expression of TXNIP protein was detected by streptavidin peroxidase (SP) and Western blot,and quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-135a-5p.Results The positive expression rate of TXNIP protein in adjacent tissues of PTC patients was significantly higher than that in tumor tissues (P<0.05);the expression level of TXNIP protein in tumor tissues of PTC patients was significantly lower than that in adjacent tissues (P<0.05);the expression of miR-135a-5p in PTC patients was significantly lower than that in adjacent tissues(P<0.05);in patients with PTC tumor diameter (≥2 cm) and lymph node metastasis,the proportion of patients with low expression of miR-135a-5p was significantly higher than those with high expression of miR-135a-5p(P<0.05);in patients with PTC tumor diameter (≥2 cm) and lymph node metastasis,the proportion of patients with negative TXNIP expression was significantly higher than that of patients with positive TXNIP expression (P<0.05);there was a significant positive correlation between the expression of miR-135a-5p and TXNIP in PTC patients (P<0.05).Conclusions The expression levels of miR-135a-5p and TXNIP in tumor tissues of PTC patients are lower than those in adjacent tissues.The expression levels of miR-135a-5p and TXNIP were positively correlated with the increase of tumor diameter and lymph node metastasis.The low expression of miR-135a-5p and TXNIP may be related to the occurrence and deterioration of PTC tumors.

关 键 词：微RNAS 载体蛋白质类 乳头状瘤 甲状腺肿瘤 

分 类 号：R736.1[医药卫生—肿瘤]