电针对睾酮低下老年大鼠睾丸间质细胞抗氧化功能的影响  

Electroacupuncture intervention increases testosterone level of aged rats by activating ERK/Nrf2/HO-1 signaling of Leydig cells

作　　者：曾婷婷 姚太万 李学智[1] 唐成林[1] ZENG Ting-ting;YAO Tai-wan;LI Xue-zhi;TANG Cheng-lin(Key Laboratory of Traditional Chinese Medicine for Pre‐vention and Cure of Metabolic Diseases,College of Traditional Chinese Medicine of Chongqing Medical University,Chongqing400016,China;Department of Rehabilitation,Affiliated Rehabilitation Hospital of Chongqing Medical University,Chongqing400050)

机构地区：[1]重庆医科大学中医药学院,中医药防治代谢性疾病重庆市重点实验室,重庆400016 [2]重庆医科大学附属康复医院康复治疗科,重庆400050

出　　处：《针刺研究》2019年第8期566-570,582,共6页Acupuncture Research

基　　金：国家自然科学基金项目（No.81303036）;重庆市基础科学与前沿技术研究项目（No.cstc2017jcyjAX0213）;重庆市渝中区科技计划项目（No.20160108）

摘　　要：目的:观察电针对睾酮低下老年大鼠睾丸间质(Leydig)细胞抗氧化功能的影响,探讨电针抗雄性生殖衰老的可能作用机制。方法:SD老年大鼠随机分为老年对照组、药物组、电针组,每组6只,SD青年大鼠6只作为青年对照组。青年对照组和老年对照组予以腹部皮下注射0.9%氯化钠溶液(7 mg·kg^-1·3d^-1);药物组给予腹部皮下注射丙酸睾酮注射液(7 mg·kg^-1·3 d^-1);电针组取"肾俞""关元"穴予电针治疗,每日1次,每次15 min,治疗5 d休息2 d,均治疗8周。采用ELISA法检测大鼠血清总睾酮(TT)和游离睾酮(FT)含量,免疫组织化学法检测Leydig细胞磷酸化细胞外信号调节蛋白激酶(p-ERK)蛋白表达水平,Western blot法检测大鼠睾丸细胞外信号调节蛋白激酶(ERK)、p-ERK、核因子E2相关因子2(Nrf2)含量及血红素氧合酶-1(HO-1)蛋白表达的变化。结果:与青年对照组比较,老年对照组血清TT、FT含量及睾丸ERK、p-ERK、Nrf2、HO-1蛋白表达水平均显著降低(P<0.01)。治疗后,电针组与药物组血清TT、FT含量及睾丸ERK、p-ERK、Nrf2、HO-1蛋白表达水平较老年对照组均显著升高(P<0.01);且电针组睾丸ERK、p-ERK、Nrf2、HO-1蛋白表达水平均显著高于药物组(P<0.01)。结论:电针可能通过调控抗氧化应激通路ERK/Nrf2,提高老年大鼠睾丸组织抗氧化功能,降低氧化应激损伤,从而改善睾酮低下老年大鼠血清TT及FT含量,这可能是电针改善雄性生殖衰老的机制之一。Objective To observe the effect of electroacupuncture(EA)on anti-oxidant function of Leydig cells in aged rats with low testosterone,so as to reveal its underlying mechanism of anti-aging of male reproduction.Methods Eighteen 20 months old SD rats were divided into aged control,medication and EA groups(n=6 in each group),and other6 young male SD rats(2 months of age)were used as the youth control group.The rats of the youth and the aged control groups received subcutaneous injection of 0.9% normal saline(7 mg·kg^-1·3 d^-1)for 8 weeks,and those of the medication group received abdominal subcutaneous injection of Testosterone Propionate(7 mg·kg-1·3 d-1) for 8 weeks.EA(2 Hz/100 Hz,1 mA)was applied to bilateral"Shenshu"(BL23)and"Guanyuan"(CV4)for 15 min,once daily for 8 weeks except the weekends.The levels of serum total testosterone(TT)and free testosterone(FT)were determined by enzyme linked immune sorbent assay(ELISA),the immunoactivity of phosphorylated extracellular signalregulated protein kinase(p-ERK)was detected by immunohistochemistry,and the expression levels of ERK,p-ERK,nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)proteins in the testis tissues were determined by Western blot.Results Before and after the treatment,the levels of serum TT and FT in the aged control group were significantly lower than those of the youth control group(P<0.01).After the treatment,the serum TT and FT contents in the EA and medication groups were obviously higher than those in the aged control group(P<0.01).Compared with the youth control group,the expression levels of ERK,p-ERK,Nrf2 and HO-1 proteins were significantly down-regulated in the aged control group(P<0.01),while in comparison with the aged control group,the expression levels of ERK,p-ERK,Nrf2 and HO-1 proteins were significantly up-regulated in the medication and EA groups(P<0.01).The therapeutic effect of EA was notably superior to that of medication in up-regulating the immunoactivity of p-ERK,and the expression levels of ERK,p-ERK

关 键 词：电针 男性生殖衰老 睾丸间质细胞 抗氧化功能 

分 类 号：R245.97[医药卫生—针灸推拿学]