不同冲洗方法对粪肠球菌感染根管的抗菌性研究  

Antibacterial efficacy of different irrigation methods on Enterococcus faecalis infected root canals in vitro

作　　者：王雅丽[1] 罗丹 申婷[1] 白新娜 陈敏慜[1] 谢晓莉[1] Wang Yali;Luo Dan;Shen Ting;Bai Xinna;Chen Minmin;Xie Xiaoli(Endodontics of Xiangya Stomatological Hospital of Central South University,Changsha 410000,China;Endodontics of Stomatological Hospital of Changsha,Changsha 410004,China)

机构地区：[1]中南大学湘雅口腔医院牙体牙髓科,长沙410000 [2]湖南省长沙市口腔医院牙体牙髓科,410004

出　　处：《中华口腔医学研究杂志（电子版）》2019年第4期204-211,共8页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：湖南省卫生计生委科研计划(C20180897)~~

摘　　要：目的比较超声、Er,Cr:YSGG激光及Er:YAG激光辅助1%次氯酸钠(NaOCl)溶液冲洗对人离体牙根管表面粪肠球菌生物膜及根尖区不同深度牙本质小管内粪肠球菌的杀灭效果。方法单直根管下颌前磨牙42颗,建立粪肠球菌生物膜感染的根管模型,采用随机字表法随机抽取2颗确定建成粪肠球菌生物膜感染根管模型,剩余40颗牙采用随机数字表法按随机对照原则分为5组,每组8颗。A组:5.25%NaOCl联合17%乙二胺四乙酸(EDTA)注射器冲洗;B组:0.9%氯化钠溶液注射器冲洗;C组:1%NaOCl溶液超声荡洗;D组:1%NaOCl溶液Er:YAG激光活化冲洗;E组:1%NaOCl溶液Er,Cr:YSGG激光活化冲洗。按分组进行处理后取样菌落计数,计算灭菌率。使用SPSS17.0统计软件对实验数据进行方差齐性及正态性检验比较各组间和组内的灭菌率。结果(1)组间比较:对根管壁表面,各组冲洗方法灭菌率比较,B组(5.74%)<C组(69.70%)<D组(90.14%)<E组(99.25%),均小于A组(阳性对照组99.95%),各组间比较差异均具有统计学意义(P<0.05);(2)组内比较:对根尖4mm不同深度牙本质相对灭菌率比较,A组和C组不同深度抗菌率比较差异均具有统计学意义(P<0.05)。结论超声荡洗、Er:YAG激光、Er,Cr:YSGG激光均可增强根管消毒效果,而两种激光根管消毒效果差异无统计学意义,均优于1%NaOCl溶液超声荡洗。Objective To compare the ultrasonic,Er,Cr:YSGG laser and Er:YAG laser.assisted 1% sodium hypochlorite(NaOCl)solution on the bactericidal effect of Enterococcus faecalis(E.faecalis) biofilm on the root canal surface and E.faecalis in different depth from dentinal tubules in the apical region. Methods Forty.two single mandibular premolars were used to establish a root canal model of E.faecalis biofilm infection. Two teeth were determined by digital randomization method to establish the model of infection of E.faecalis. The remaining 40 teeth were divided into 5 groups according to the principle of random comparison by digital random method as well as 8 in each group. The groups were:Positive Control:group A:5.25% NaOCl + 17% EDTA springed,Negative Control:group B:physiologic saline springed,group C:ultrasound+1% NaOCl,group D:Er:YAG laser+1% NaOCl,group E:Er,Cr:YSGG+ 1% NaOCl. The data from each group were compared using the SPSS 17.0 statistical software for the homogeneity of the experimental data and the normality test to compare the relative sterilization rates between groups and groups. Results (1)Comparison between groups:The sterilization rate of each group was compared. Group B(5.74%)<C group(69.70%)<D group(90.14%)<E group(99.25%),All of which were lower than group A(99.95% of the positive control group),and the differences between the groups were statistically significant(P<0.05).(2)Comparison within the group:Compared with the relative sterilization rate of dentin at different depths of 4 mm,the difference of antibacterial rate in different depths of group A and group C was statistically significant(P<0.05). Conclusions Ultrasound, Er:YAG laser,Er,Cr:YSGG laser can enhance the root canal disinfection effect,and the difference between the two laser root canal disinfection effects is not statistically significant,both of which are better than 1% NaOCl ultrasonic washing.

关 键 词：根管冲洗剂 激光疗法 超声疗法 激光活化冲洗 超声荡洗 

分 类 号：R781.05[医药卫生—口腔医学]