长链非编码RNA ITGβ2-AS1对胰腺癌细胞增殖和侵袭及迁移能力的影响  

Effect of Long Non-coding RNA ITGβ2-AS1 on Proliferation, Invasion and Migration of Pancreatic Cancer Cells

作　　者：陈世裕 喻超[2,3,4] 潘耀振 陈玲[2,3,4] 李琳 杨哲豪[1,2,3,4] 吕彦霖 邓路 孙诚谊 CHEN Shiyu;YU Chao;PAN Yaozhen;CHEN Ling;LI Lin;YANG Zhehao;LV Yanlin;DENG Lu;SUN Chengyi(Guizhou Medical University, Guiyang 550004, Guizhou, China;Department of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China;Key Laboratory of Liver, Gallbladder, Pancreasand Spleen of Guizhou Medical University, Guiyang 550004, Guizhou, China;Guizhou Institute of Hepatobiliary, Pancreatic and Spleen Diseases, Guiyang 550004, Guizhou, China)

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州医科大学附院肝胆外科,贵州贵阳550004 [3]贵州医科大学肝胆胰脾重点实验室,贵州贵阳550004 [4]贵州省肝胆胰脾疾病研究所,贵州贵阳550004

出　　处：《贵州医科大学学报》2019年第8期869-874,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81860505,81860506);贵州省科学技术厅—贵州医科大学附属医院联合基金[黔科合LH字(2016)7229];贵州省肝胆外科临床医学研究中心[黔科合平台人才(2017)5404];贵州省高层次创新人才培养计划“十”层次人才,黔科合平台人才[(2016)5647];贵州省科学技术厅—贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013];贵州省孙诚谊“肝胆胰脾疾病诊治”导师工作室,黔教研合[GZS(2016)09];贵州省第四批人才基地—贵州省外科人才培养基地[黔省专合字(2012)94];贵州省科技支撑计划项目[黔科合SY字(2015)3047]

摘　　要：目的:探讨长链非编码RNA(LncRNA)整合素β2(ITGβ2)-AS1在体外对人胰腺癌细胞增殖、侵袭及迁移能力的影响及其机制。方法:采用癌症基因组图谱(TCGA)公共数据库分析ITGβ2-AS1在胰腺癌组织及癌旁组织中的表达,通过qPCR实验检测ITGβ2-AS1分别在人正常胰腺导管上皮细胞及人PC细胞株AsPC-1、CFPAC-1、PANC-1、MIAPaCa-2、Capan-2、BxPC-3、Hs766T及SW1990细胞中的表达,利用慢病毒载体稳定转染ITGβ2-AS1最高的表达细胞,采用CCK-8和平板克隆实验检测ITGβ2-AS1对PANC-1细胞增殖能力的影响;通过细胞划痕及Transwell实验检测ITGβ2-AS1对PANC-1细胞侵袭及迁移能力的影响,qPCR及Westernblot实验检测干扰ITGβ2-AS1后PANC-1细胞中ITGβ2的基因表达情况。结果:ITGβ2-AS1在胰腺癌组织中的表达明显高于癌旁组织,且在各胰腺癌细胞株中的表达显著高于人正常胰腺导管上皮细胞,PANC-1中表达最高,差异有统计学意义(P<0.05);干扰ITGβ2-AS1后,PANC-1细胞的增殖、迁移及侵袭能力显著降低,ITGβ2的表达水平显著下降,差异有统计学意义(P<0.05)。结论:LncRNAITGβ2-AS1在胰腺癌中高表达,干扰其表达可抑制胰腺癌细胞的增殖、侵袭及迁移能力和抑制ITGβ2的转录及翻译过程。Objective: To investigate the effect of long non-coding RNA integrin β2 (ITGβ2)-AS1 on proliferation,invasion and migration of human pancreatic cancer cells in vitro and to explore its mechanism preliminarily. Methods: The expression of ITGβ2-AS1 in pancreatic cancer tissues and adjacent tissues was analyzed and compared based on the TCGA database and the expression of ITGβ2-AS1 in human normal pancreatic ductal epithelial cells and pancreatic cancer cells was detected by qPCR assay;The pancreatic cancer cell line PANC-1 was stably transfected with lentiviral vector and the effect of ITGβ2-AS1 on proliferation of pancreatic cancer cells was detected by CCK-8 and colony formation assays;The effect of ITGβ2-AS1 on invasion and migration of pancreatic cancer cells was detected by wound healing and Transwell assays;The expression of ITGβ2 after interfering with ITGβ2-AS1 was detected by qPCR and Western blot assays. Results: The expression of ITGβ2-AS1 in pancreatic cancer tissues was significantly higher than that in adjacent tissues,and the expression of ITGβ2-AS1 in pancreatic cancer cell lines was significantly higher than that in human normal pancreatic ductal epithelial cells ( P <0.05). After interfering with ITGβ2-AS1,the proliferation,migration and invasion ability of pancreatic cancer cells decreased significantly ( P <0.05) and the expression level of ITGβ2 decreased significantly ( P <0.05). Conclusion: Long non-coding RNA ITGβ2-AS1 is highly expressed in pancreatic cancer,interfering its expression may inhibit the proliferation,invasion and migration of pancreatic cancer cells and inhibit the transcription and translation process of ITGβ2.

关 键 词：长链非编码RNA 整合素β2 胰腺癌 增殖 侵袭 迁移 

分 类 号：R735.9[医药卫生—肿瘤]