腺花素通过靶向Prx Ⅲ诱导急性早幼粒白血病细胞分化的研究  

Adenanthin Induces Differentiation of Acute Promyelocytic Leukemia Cells by Targeting Peroxiredoxin Ⅲ

作　　者：莫运喜[1] 刘丹莉[1] 高顺利[1] 殷小成[1] MO Yun-Xi;LIU Dan-Li;GAO Shun-Li;YIN Xiao-Cheng(Department of Pediatrics, The First Hospital Affiliated to University of South China, Hengyang 421001, Hunan Province, China)

机构地区：[1]南华大学附属第一医院儿科

出　　处：《中国实验血液学杂志》2019年第4期1083-1087,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(31271482)

摘　　要：目的:探讨腺花素(Adenanthin)靶向PrxⅢ诱导急性早幼粒白血病(acute promyelocyticleukemia,APL)细胞分化。方法:以HL-60细胞株作为研究对象,取对数生长期细胞,分为对照、全反式维甲酸(ATRA)、Adenanthin和ATRA+Adenanthin,共4组。观察各组细胞形态的变化;用NBT还原实验分析细胞分化能力的变化;流式细胞术检测细胞表面分化抗原CD11b的表达变化;采用Western blot检测PrxⅢ表达的变化。结果:Adenanthin可诱导HL-60细胞分化;ATRA+Adenanthin组NBT还原阳性率明显高于ATRA组和Adenanthin组(P<0.05);ATRA+Adenanthin组CD11b阳性细胞百分率(43.62%±1.38%)均明显高于Adenanthin组(28.15%±1.78%)、ATRA组(36.72%±1.33%)及空白对照组(7.99%±1.78%)(P<0.05);Adenanthin组PrxⅢ蛋白水平明显高于空白对照组及ATRA组(P<0.05)。结论:腺花素能协同ATRA使HL-60细胞分化成熟,其作用机制可能与调控PrxⅢ的表达有关。Objective:To investigate the differentiation of acute promyelocytic leukemia (APL) cells induced by adenosine targeting Prx Ⅲ. Methods:HL-60 cells were divided into four groups:control group, all-trans retinoic acid (ATRA) group, adenanthin group and ATRA+adenanthin group. Cell morphologic changes were observed under optical microscope. The influence of adenanthin on the differentiation of HL-60 was observed by nitro blue tetrazolium chloride (NBT) test. Cell surface differentiation antigens CD11b expression was measured by flow cytometry. The protein expression of Prx Ⅲ was detected by immunohistochemical assay. Results:Adenanthin could induce the differentiation of HL-60 cells;the NBT reduction positive rate in ATRA+adenanthin group was significantly higher than that in ATRA group and adenanthin group (P < 0.05). The percentage of CD11b positive cells in ATRA+adenanthin group (43.62%±1.38%) was higher than that in adenanthin group (28.15%±1.78%), ATRA group (36.72%±1.33%) and control group (7.99%±1.78%)(P < 0. 05). The content of Prx Ⅲ protein in adenanthin group was significantly higher than that in control group and ATRA group (P < 0.05). Conclusion:Adenanthin and ATRA have a synergistic effect on the differentiation and maturation of HL-60 cells, and its mechanism may be related with regulation of Prx Ⅲ expression.

关 键 词：腺花素 PrxⅢ 急性早幼粒白血病 

分 类 号：R733.71[医药卫生—肿瘤]