不同方法处理的浒苔的小鼠体内抗氧化活性比较  

Comparison of Antioxidation Activities in a Mouse Model for Enteromorpha Processed with Different Methods

作　　者：董晓静 王鹏 伍彬[2] DONG Xiao-jing;WANG Peng;WU Bin(Department of Food Engineening, Weihai Ocean Vocational College, Weihai 264300, China;College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025, China)

机构地区：[1]威海海洋职业学院食品工程系,山东威海264300 [2]广东海洋大学食品科技学院,广东湛江524025

出　　处：《现代食品科技》2019年第8期73-77,12,共6页Modern Food Science and Technology

基　　金：威海海洋职业学院校级项目(KY201802)

摘　　要：本研究比较了三种不同方法处理的浒苔在小鼠体内的抗氧化作用,在衰老模型小鼠灌胃给予浒苔原粉(EO),浒苔多糖(EP),浒苔超声处理粉末(EU-L)低剂量,浒苔超声处理粉末(EU-H)高剂量分别为300 mg/kg·d,100 mg/kg·d,300 mg/kg·d,350 mg/kg·d,7周后检测其对小鼠血清、肝脏、脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、过氧化氢酶(CAT)含量的影响。结果表明,浒苔产品对小鼠体重增长无影响;其中,血清中CAT含量由模型组的6.90U/m L提高到EP组8.79U/m L,EU-L组8.31 U/mL,EU-H组9.43 U/mL,与模型组对照差异极显著(p<0.01),EU-H组小鼠血清、肝和脑组织的SOD、GSH-Px、CAT含量均显著高于模型组(p<0.01);给予浒苔产品的四组小鼠MDA含量均低于模型组9.94U/m L,7.88U/mg prot,6.63U/mg prot(p<0.05)。说明,浒苔超声粉高剂量组(EU-H)能够有效提高衰老模型小鼠体内抗氧化酶活性,对衰老小鼠的治愈率达90%以上,效果优于浒苔多糖(EP);而且根据剂量比较,浒苔超声粉(EU)可以剂量依赖性地提高衰老小鼠体内抗氧化能力。This study compared the antioxidant effects of three Enteromorpha products processed with different processing methods in a mouse model. Aged mice were administrated by gavage with enteromorpha original (EO, 300 mg/kg.d), enteromorpha polysaccharide (EP, 100 mg/kg.d), low dose of enteromorpha ultrasound (EU-L, 300 mg/kg.d) or high dose of enteromorpha ultrasound (EU-H, 350 mg/kg.d). The contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), catalase (CAT) in serum, liver and brain tissues of mice were analyzed after 7 weeks. The results showed that all these enteromorpha products had no effect on the weight gain of the mice. The serum CAT content in model group was 6.90 U/mL, which was significantly (p<0.01) lower than to those for the EP group (8.79 U/mL), EU-L group (8.31 U/mL) and EU-H group (9.43 U/mL). The contents of SOD, GSH-Px and CAT in serum, liver and brain tissues of the EU-H were significantly (p<0.01) higher than the model group. The MDA contents of the four groups fed with enteromorpha products were lower (p<0.05) than that of the model group:9.94 U/mL, 7.88 U/mg prot, 6.63 U/mg prot (p<0.05).These results indicate that EU-H effectively improved the activities of antioxidant enzymes in the aged mouse model with the rate of reversing aging over 90%,showing advantages over EP;According to the comparison on dose, EU could increase the antioxidant effect on aged mice in a dose-dependent manner.

关 键 词：浒苔 抗氧化 超氧化物歧化酶 

分 类 号：S816.7[农业科学—饲料科学]