青稞再生体系的建立及优良受体基因型筛选  

Establishing a Hulless Barley Regeneration System and Selecting Fine Hulless Barley Genotypes

作　　者：徐齐君 王玉林[1,2] 原红军 巴桑玉珍[1,2] 扎桑 于明寨[1,2] 杨春葆 尼玛扎西[1,3] XU Qi-jun;WANG Yu-lin;YUAN Hong-jun;YUZHEN Ba-sang;ZHA Sang;YU Ming-zhai;YANG Chun-bao;NYIMA Ta-shi(State Key Laboratory of Hulless Barley and Yak GermplasmResources and Genetic Improvement , Lhasa 850002, China;Institute of Agricultural Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China;Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China)

机构地区：[1]省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,西藏拉萨850002 [2]西藏自治区农牧科学院农业研究所,西藏拉萨850002 [3]西藏自治区农牧科学院,西藏拉萨850002

出　　处：《大麦与谷类科学》2019年第4期1-10,38,共11页Barley and Cereal Sciences

基　　金：西藏财政专项（XZNKY-2018-C-021、2017CZZX001）

摘　　要：为了筛选出高频再生能力强的青稞基因型,对青稞成熟胚处理方式、愈伤诱导、胚性愈伤诱导和分化的培养基成分进行了研究。结果表明,碎胚携带胚乳更利于青稞愈伤组织的发生。不同青稞品种在不同培养基上的愈伤诱导情况存在显著差异。供试材料中只有青稞昆仑14号、ZYM0439和C1可诱导得到淡黄色、致密的胚性愈伤,且昆仑14号和ZYM0439可获得高频的次级胚性愈伤。由于C1在初生愈伤诱导的时候很容易出芽和水渍化,所以次级胚性愈伤诱导率较低。在不同的分化培养基上,青稞ZYM0439的分化能力明显优于青稞昆仑14号,更适宜用作青稞遗传转化研究的材料。The current research aimed at selecting hulless barley genotypes with a high regeneration rate of mature embryos.Thus,we studied the methods of treating hulless barley mature embryos;and the ingredients of media for callus induction,embryo callus induction,and differentiation.The results showed that the broken embryo with endosperm was more conducive to the development of hulless barley callus than the one without endosperm.Different hulless barley varieties showed significant differences in callus induction on different culture media.Among all tested hulless barley varieties,only ’Kunlun 14’,’ZYM0439’,and ’C1’ generated pale yellow and dense embryogenic callus.Besides,high-frequency secondary embryogenic callus was induced on ’Kunlun 14’ and’ZYM0439’.For ’C1’,it was easy to sprout and form water stains during the induction of primary callus,which resulted in a low rate of generating secondary embryogenic callus.On different differentiation culture media,the differentiation ability of ’ZYM0439’ was significantly stronger than that of ’Kunlun 14’,so ’ZYM0439’ is more suitable for the research of genetic transformation in hulless barley.

关 键 词：青稞 成熟胚 愈伤组织 诱导 植株再生 

分 类 号：S512.3[农业科学—作物学]