氧化型低密度脂蛋白与急性冠状动脉综合征患者CD4^+T细胞Notch1、Notch2表达的相关性研究  

Association among oxidized low density lipoprotein and the expression of Notch1,Notch2 in CD4+T cells in patients with acute coronary syndrome

作　　者：朱家平 朱李茹[1] ZHU Jiaping;ZHU Liru(Department of Medicine, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science,Xiangyang 441021,China)

机构地区：[1]襄阳市中心医院湖北文理学院附属医院医学检验部

出　　处：《天津中医药》2019年第9期865-868,共4页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]探讨氧化型低密度脂蛋白(ox-LDL)与急性冠状动脉综合征(ACS)患者CD4^+T细胞Notch1、Notch2表达的相关性研究。[方法]纳入健康对照人群30例,稳定型心绞痛(SA)患者32例,ACS患者56例[不稳定型心绞痛(UA)患者30例,急性心肌梗死(AMI)26例],收集外周血标本,酶联免疫吸附测定(ELISA)试剂盒检测ox-LDL表达,流式细胞仪检测CD4^+T细胞Notch1、Notch2分子的表达变化及Th1/Th2比值改变。采用流式细胞术分选CD4^+T细胞,利用低、中、高剂量ox-LDL(0、40、80、160 mg/L)刺激细胞72 h,利用流式细胞仪检测CD4^+T细胞Notch1、Notch2分子的表达变化。[结果] UA组、AMI组CD4^+T细胞Notch1、Notch2表达明显降低,而Th1/Th2比值及ox-LDL水平明显升高(P<0.05)。经Pearson相关性分析发现,ACS患者(UA组、AMI组)外周血ox-LDL与Notch1(r=-0.653,P<0.01)、Notch2(r=-0.468,P<0.01)表达呈明显负相关,与Th1/Th2比值呈明显正相关(r=0.582,P<0.01)。低、中、高剂量ox-LDL可明显阻滞Notch1、Notch2表达(P<0.05)。[结论] ox-LDL能明显抑制人外周血CD4^+T细胞Notch1、Notch2分子表达,可能与ox-LDL抑制Th2分化而促进Th1异常分化有关。[Objective] To study the association between oxidized low density lipoprotein(ox-LDL) and the expression of Notch1,Notch2 in CD4^+T cells in patients with acute coronary syndrome.[Methods] The 26 patients with acute myocardial infarction(AMI),30 patients with unstable angina(UA),32 patients with stable angina(SA) and 30 healthy subjects(NC) were recruited. The plasma level of oxLDL was measured by ELISA,and the ratio of Th1/Th2 and the expression of Notch1,Notch2 in CD4^+T cells in peripheral blood were detected by flow cytometry. Then CD4^+T cells were sorted by flow cytometry,T cells were cocultured with varying concentrations of oxLDL(0,40,80,160 mg/L),the expression of Notch1 and Notch2 in CD4^+T cells were detected by flow cytometry in each group after 72 h.[Results] The expression levels of Notch1,Notch2 in CD4^+T cells were greatly decreased,the ratio of Th1/Th2 and the level of ox-LDL were significantly increased in AMI group and UA group compared with NC group(P<0.05). Pearson correlation analyses indicated that ox-LDL was significantly and negatively associated with the expression of Notch1(r=-0.653,P<0.01) and Notch2(r=-0.468,P<0.01) in patients with acute coronary syndrome,and ox-LDL was significantly and positively associated with the ratio of Th1/Th2(r=0.582,P<0.01).[Conclusions] ox-LDL can obviously inhibit the expression of Notch1 and Notch2 in human peripheral blood CD4^+T cells,which may be the reason that ox-LDL inhibit the differentiation of Th2 and promote the differentiation of Th1,and participate in the occurrence and development of atherosclerosis.

关 键 词：急性冠状动脉综合征 氧化型低密度脂蛋白 TH1/TH2细胞 NOTCH1 NOTCH2 

分 类 号：R541.4[医药卫生—心血管疾病]