中华地鳖虫肠道内生真菌的分离鉴定及抑菌活性研究  

Isolation and Identification of Intestinal Endophytic Fungi from Eupolyphaga sinensis and Its Antibacterial Activity

作　　者：李娜[1] 李凡 胡高升[1] 赵玥[1] 贾景明[1] LI Na;LI Fan;HU Gao-sheng;ZHAO Yue;JIA Jing-ming(School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学中药学院

出　　处：《中国现代中药》2019年第9期1246-1250,共5页Modern Chinese Medicine

基　　金：辽宁省教育厅项目(201610163L19)

摘　　要：目的:研究中华地鳖虫肠道内生真菌及其抑菌活性,为发现新型活性天然产物提供一条重要的途径。方法:采用平板分离法从地鳖虫肠道内分离内生真菌,利用滤纸片法对其进行抑菌活性研究,并对菌株的总DNA进行提取,利用通用引物ITS1和ITS4对菌株18S rDNA ITS序列进行扩增和测序,将测序结果进行同源性比对分析,确定活性菌株的分类地位。结果:从中华地鳖虫肠道中共分离出8株内生真菌。其中,菌株DB-1对金黄色葡萄球菌、枯草芽孢杆菌和白色念珠菌都有一定的抑制作用,经比对分析后鉴定菌株DB-1为深绿木霉Trichoderma atroviride。结论:中华地鳖虫肠道内具有抑菌活性的内生真菌资源可做进一步研究利用。Objective: To study the intestinal endophytic fungi from Eupolyphaga sinensis and their antibacterial activity in order to provide an important way for discovery of new active natural products. Methods: Endophytic fungi were isolated from the intestines of E.sinensis by using the method of plate cultivation,and the antibacterial activity of the fermented metabolites was studied by the scrip diffusion method.The total DNA were extracted,and the 18S rDNA ITS were amplified and sequenced with primer ITS1 and ITS4,then the results of sequencing were analyzed comparatively based on homology to confirm the classification of active strains. Results: 23 strains of endophytic fungi were isolated.The strain DB-1 had inhibitory effect on Staphylococcus aureus , Bacillus subtilis and Candida albicans .The DB-1 was identified as Trichoderma atroviride by comparative analysis. Conclusion: The results showed that the intestines of E.sinensis contain abundant endophytic fungi resources with antibacterial activity,which could be further developed and utilized.

关 键 词：中华地鳖虫 肠道内生真菌 抑菌活性 

分 类 号：R282.74[医药卫生—中药学] R284[医药卫生—中医学]