促肝细胞再生磷酸酶-3在鼻腔鼻窦鳞癌中的表达及与预后的关系研究  

Expression of phosphatase of regenerating liver-3 in sinonasal squamous cell carcinoma and its relationship with prognosis

作　　者：张寅斌[1] 陈银溪 马宇光[1] 刘棣 梁亮[1] 王梦[1] 孙诗雨 张淑群[1] ZHANG Yin-bin;CHEN Yin-xi;MA Yu-guang(Department of Oncology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an Shaanxi 710000,China.)

机构地区：[1]西安交通大学第二附属医院肿瘤科

出　　处：《临床和实验医学杂志》2019年第18期1964-1967,共4页Journal of Clinical and Experimental Medicine

基　　金：陕西省科技攻关项目(编号:2015SF083)

摘　　要：目的研究促肝细胞再生磷酸酶-3(PRL-3)在鼻腔鼻窦鳞癌中的表达及与临床病理特征的关系。方法采用回顾性研究,收集54例鼻腔鼻窦鳞癌患者(鼻腔鼻窦鳞癌组)、36例鼻息肉患者(鼻息肉组)及30例正常人(对照组)鼻腔黏膜组织标本。采用免疫组织化学染色法对其PRL-3蛋白表达阳性率进行检测,并用实时荧光定量PCR法对PRL-3 mRNA相对表达量进行检测,同时分析PRL-3与鼻腔鼻窦鳞癌患者临床病理特征的相关性。结果鼻腔鼻窦鳞癌组PRL-3蛋白表达阳性率较对照组、鼻息肉组明显升高(P <0. 05)。与对照组、鼻息肉组相比,PRL-3mRNA在鼻腔鼻窦鳞癌组中的表达明显上调(P <0. 05)。不同性别、年龄的鼻腔鼻窦鳞癌患者PRL-3蛋白表达阳性率的比较,并无显著差异(P> 0. 05);低分化、病理分期为Ⅲ~Ⅳ期、伴有淋巴结转移的鼻腔鼻窦鳞癌患者PRL-3蛋白表达阳性率明显高于高分化、病理分期为Ⅰ~Ⅱ期、无淋巴结转移者(P <0. 05)。结论 PRL-3高表达于鼻腔鼻窦鳞癌患者,且与肿瘤分化程度、病理分期及是否伴有淋巴结转移密切相关,提示其有望成为评估鼻腔鼻窦鳞癌患者预后的独立预测因子。Objective To study the expression of phosphatase of regenerating liver-3( PRL-3) in sinonasal squamous cell carcinoma and its relationship with clinicopathological features. Methods A retrospective study was conducted to collect 54 cases of sinonasal squamous cell carcinoma( sinonasal squamous cell carcinoma group),36 cases of nasal polyps( nasal polyps group) and 30 cases of normal nasal mucosa( control group). Immunohistochemical staining was used to detect the positive rate of PRL-3 protein expression,and real-time quantitative PCR was used to detect the relative expression of PRL-3 mRNA. The correlation between PRL-3 and clinicopathological features of sinonasal squamous cell carcinoma was analyzed. Results The expression of PRL-3 protein in sinonasal squamous cell carcinoma group was significantly higher than that in control group and nasal polyp group( P < 0. 05). Compared with the control group and nasal polyp group,the expression of PRL-3 mRNA in sinonasal squamous cell carcinoma group was significantly up-regulated( P < 0. 05). There was no significant difference in the positive rate of PRL-3 protein expression between patients of different gender and age( P > 0. 05). The positive rate of PRL-3 protein expression in patients with poorly differentiation,stage III-IV and lymph node metastasis in patients with sinonasal squamous cell carcinoma was significantly higher than that in patients with highly differentiation,pathological stage I-II,and no lymph node metastasis( P < 0. 05). Conclusion PRL-3 is highly expressed in patients with sinonasal squamous cell carcinoma,and is closely related to the degree of differentiation,pathological stage and lymph node metastasis,suggesting that PRL-3 may be an independent predictor of the prognosis of patients with sinonasal squamous cell carcinoma.

关 键 词：鼻腔鼻窦鳞状细胞癌 促肝细胞再生磷酸酶-3 表达 预后 

分 类 号：R739.62[医药卫生—肿瘤]