猪细小病毒NJ株ST细胞适应性研究  

Study on the Adaptation of Porcine Parvovirus to ST Cell Line

作　　者：唐波[1,2,3,4] 刘国阳 常晨[1,2,3,4] 华涛 张道华[1,2,3,4] TANG Bo;LIU Guo-yang;CHANG Chen(Institute of Veterinary Immunology Engineering,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014;National Research Center of Engineering and Technology for Veterinary Biologicals,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014;Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base,Ministry of Science and Technology,Nanjing,Jiangsu 210014;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009)

机构地区：[1]江苏省农业科学院动物免疫工程研究所,江苏南京210014 [2]江苏省农业科学院,国家兽用生物制品工程技术研究中心,江苏南京210014 [3]江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,江苏南京210014 [4]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《安徽农业科学》2019年第18期93-94,110,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(17)3049]

摘　　要：为评价ST细胞代次的增高对猪细小病毒NJ株免疫原性的影响,将第10、30、60代ST细胞分别接种猪细小病毒NJ株F8 、F18代毒种进行病毒培养,通过检测其病毒含量、血凝价以及制备疫苗免疫机体的抗体水平等指标来进行评价。结果表明,各代次病毒含量为10 7.2 ~10 7.5 TCID 50 /mL,血凝价为2 9~2 10;制苗后免疫阴性豚鼠与后备母猪,免疫28 d后均出现抗体反应,HI效价分别为2 7~2 9和2 8~2 9。不同代次毒种接种不同代次ST细胞培养的病毒含量、血凝价及疫苗免疫效力均符合要求。各代次细胞增殖性能稳定,培养的PPV NJ株均能满足兽用生物制品的生产要求。In order to evaluate the effects of generation change of swine testicle cell on the immunogenicity of NJ strain of porcine parvovirus,the 10 th ,30 th and 60 th generations of ST cells were inoculated with F8 and F18 virus of NJ strain of porcine parvovirus and cultured.And the virus content,hemagglutination titer and antibody level of vaccine immune were detected.The results showed the virus content of different generations was 10 7.2 -10 7.5 TCID 50 /mL,the haemagglutinin titer was 2 9-2 10 .The negative guinea pigs and prepubertal sows were immunized with the prepared vaccine,antibody response appeared after immunization 28 days,and HI titer were 2 7-2 9 and 2 8-2 9 respectively.The virus titer,hemagglutination titer and immune efficacy of inoculation with different generations of ST cell were in accordance with the requirements.All generations of cells had stable proliferation performance,PPV NJ strain cultured could meet the production requirements of veterinary biology product.

关 键 词：猪细小病毒 ST细胞 适应性 

分 类 号：S852.651[农业科学—基础兽医学]