响应面法优化白芷水溶性多糖提取工艺的研究  

Optimization of extraction process of water soluble polysaccharide from Angelica dahurica by response surface methodology

作　　者：唐森 农艳宁 覃逸明[2,3,4] 谢济运 吴国勇 张鹏[1,2,3] TANG Sen;NONG Yan-Ning;QIN Yi-Ming;XIE Ji-Yun;WU Guo-Yong;ZHANG Peng(School of Food and Biochemical Engineering,Guangxi Science & Technology Normal University,Laibin 546119,China;Functional Food Ingredients Engineering Research Center,Guangxi Science & Technology Normal University,Laibin 546119,China;Key Laboratory for Research and Development of Characteristic Yao Medicine Resources,Guangxi Science & Technology Normal University,Laibin 546119,China;Scientific Research Management Department,Guangxi Science & Technology Normal University,Laibin 546119,China)

机构地区：[1]广西科技师范学院食品与生化工程学院,来宾546119 [2]广西科技师范学院功能性食品配料工程技术研究中心,来宾546119 [3]广西科技师范学院特色瑶药资源研究与开发重点实验室,来宾546119 [4]广西科技师范学院科研管理处,来宾546119

出　　处：《食品安全质量检测学报》2019年第15期4937-4943,共7页Journal of Food Safety and Quality

基　　金：广西糖资源工程技术研究中心培育建设项目(桂科AD16450040);广西高校制糖工程综合技术重点实验室培育建设项目[桂教科研(2016)6号] ~~

摘　　要：目的采用响应面法优化白芷水溶性多糖的提取工艺.方法用苯酚-硫酸比色法测定白芷水溶性多糖在波长490 nm处的吸光度,计算多糖提取率并以此为实验指标,在单因素实验的基础之上,选取提取温度、提取时间、液料比为考察因素,利用Box-Bebnken方法进行三因素三水平实验设计.结果单因素最佳试验条件为液料比100∶1(mL∶g),提取时间90 min,提取温度70℃;响应面法优选出白芷水溶性多糖最佳提取工艺参数为液料比102∶1(mL∶g),提取时间90 min,提取温度68℃,在该优化条件下多糖实际提取率7.35%±0.005%.结论本研究优化的白芷水溶性多糖的提取工艺方便、稳定.Objective To optimize the extraction process of water-soluble polysaccharides from Angelica dahurica Alba by response surface methodology.Methods Phenol-sulfuric acid colorimetric method was used to determine the absorbance of water-soluble polysaccharides from Angelica dahurica at 490 nm.The extraction rate of polysaccharides was calculated as an experimental index.On the basis of single factor experiment,the extraction temperature,extraction time and liquid-material ratio were selected as the factors to investigate.Box-Bebnken method was used to design three-factor and three-level experiment.Results The optimum conditions for single factor extraction were as follows:the ratio of liquid to material was 100:1(mL:g),the extraction time was 90 min,and the extraction temperature was 70 C.Response surface methodology was used to optimize the extraction parameters of Angelica dahurica water-soluble polysaccharides:the ratio of liquid to material was 102:1(mL:g),the extraction time was 90 min,and the extraction temperature was 68℃C.Under the optimum conditions,the actual extraction rate of polysaccharides was 7.35%±0.005%.Conclusion The optimized extraction process of watersoluble polysaccharides from Radix Paeoniae Alba is convenient and stable.

关 键 词：白芷多糖 提取工艺 优化 响应面分析法 

分 类 号：TQ281[化学工程—有机化工]