过表达OsALSC512A基因拟南芥对几种除草剂抗性的研究  

Study on the Resistance of Arabidopsis Overexpressing OsALSC512A Gene to ALS Inhibitor Herbicides

作　　者：辛洁 胡健立 张红磊 高晨旭 刘菲[1] 张融雪[2] 苏京平[2] 孙玥[2] 朱晔荣[1] 刘学军[2] 王勇[1] Xin Jie;Hu Jianli;Zhang Honglei;Gao Chenxu;Liu Fei;Zhang Rongxue;Su Jingping;Sun Yue;Zhu Yerong;Liu Xuejun;Wang Yong(College of Life Sciences,Nankai University, Tianjin 300071, China;Tianjin Crop Research Institute,Tianjin 300112, China)

机构地区：[1]南开大学生命科学学院,天津300071 [2]天津市农作物研究所,天津300112

出　　处：《南开大学学报（自然科学版）》2019年第4期99-106,共8页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家重点研发计划子课题(2017YFD01005050103);天津市2018年现代农业产业技术体系创新团队建设(ITTRRS2018003)

摘　　要：以乙酰乳酸合酶(ALS)为靶标的除草剂是目前生产中应用较为广泛的除草剂,具有用量少、选择性强、杀草谱广、生物活性高,对哺乳动物毒性低的特点.本研究对水稻ALS编码基因进行了定点突变,获得了突变基因OsALSC512A,实现了第171位脯氨酸残基成为组氨酸残基(Pro171His);然后构建了其过表达载体,并转化拟南芥进行了除草剂抗性检测.对分属四类的四种除草剂的生测结果显示,突变后的水稻ALS赋予了转基因拟南芥对双草醚、甲基二磺隆和五氟磺草胺的抗性,但是没有赋予对普施特的抗性.研究结果对于进一步开展水稻抗ALS靶标除草剂的基因编辑以及获得抗除草剂新种质有一定的指导意义.ALS(acetylactate synthase)inhibitor herbicides is a class of herbicides commonly used on the market.The using dose of these kinds of herbicides is small amount,and the herbicides has more advantages,such as good selectivity,broad spectrum,high bioactivity and safe for mammal.This study obtained OsALSC512Aby performing site-directed mutagenesis in OsALS gene,and achieved Pro171 changing to His.Then expression vector was constructed and transformed into Arabidopsis,and their herbicide resistance was been analyzed.The results showed that the OsALSC512Ahomozygous line have resistance to bispyribac-sodium,mesosulfuron-methyl and penoxsulam,but no resistance to Imazethapyr.The results play an important significance in further gene editing to OsALS targeting herbicides in rice and obtaining new varieties resistance to herbicides.

关 键 词：水稻 除草剂抗性 ALS 点突变 拟南芥 

分 类 号：Q949.9[生物学—植物学]