三氯生单独暴露对斑马鱼幼鱼眼部的毒性研究  

Toxic Effect of Triclosan Exposure on the Eyes of Zebrafish Larvae

作　　者：张灵 刘秘[1] 王雯雯 叶嘉星 方周溪 王慧利[1] 周志明[2] 艾为明[1] ZHANG Ling;LIU Mi;WANG Wen-wen;YE Jia-xing;FANG Zhou-xi;WANG Hui-li;ZHOU Zhi-ming;AI Wei-ming(School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou 325000,Zhejiang,China;Zhejiang Mariculture Research Institute,Wenzhou 325000,Zhejiang,China)

机构地区：[1]温州医科大学检验医学院生命科学学院,中国浙江温州325000 [2]浙江省海洋水产养殖研究所,中国浙江温州325000

出　　处：《生命科学研究》2019年第4期290-296,共7页Life Science Research

基　　金：浙江省自然科学基金资助项目(LY15C030004);国家级大学生创新创业训练计划项目(201810343014);浙江省大学生科技创新活动计划暨新苗人才计划项目(2018R413024);温州市科技计划项目(S20170002)

摘　　要：三氯生(2,4,4’-三氯均二苯脲, triclosan, TCS)是环境中应用最为广泛的新兴外源性污染物之一,可强烈地吸附在土壤、沉积物、胶体中。TCS 具有生物蓄积作用,在生物体内的富集浓度远超过在水、土壤等环境中的浓度,并且可通过生物富集作用进入食物链。本实验以斑马鱼(Danio rerio)胚胎为模型,探讨不同浓度的TCS暴露对斑马鱼胚胎、幼鱼发育的影响。结果发现, TCS 暴露对斑马鱼胚胎和幼鱼具有较强的致畸、致死效应。为了进一步了解TCS 对斑马鱼眼部超微结构的影响,将其眼部电镜切片放在透射电镜下观察。结果显示, TCS 暴露后斑马鱼幼鱼眼部感光细胞外节盘数量明显减少,外网状层及内网状层较对照组明显变薄且空泡化严重,外核层细胞核固缩现象严重甚至部分细胞核出现溶解的现象。实验结果表明TCS 暴露对斑马鱼幼鱼眼部发育有潜在的毒性作用,这可为进一步探讨TCS 对人体潜在的毒性作用提供科学、可靠的依据。Triclosan(2,4,4’-trichloro-diphenylurea, TCS) is one of the most widely used emerging environmental contaminants in the environment, and it can be strongly adsorbed in soil, sediments, and colloids.The bioaccumulation of TCS makes its concentration in organisms far exceed those in water, soil and other environments. TCS is able to enter the food chain through bioaccumulation. Herein, zebrafish( Danio rerio)embryos were used as the model to discuss the effects of different concentrations of TCS on the development of zebrafish embryos and larvae. The results showed that TCS exposure had obviously teratogenic and lethal effects on zebrafish embryos and larvae. In order to further understand the effects of TCS on the ocular mi-crostructures of zebrafish, the sections were made and observed by transmission electron microscopy. It showed that the number of extracellular photosensitive nodes in the eyes of zebrafish larvae was significantly reduced after TCS exposure. The outer reticular and intramural layers were significantly thinner and showed more severe vacuolation than those in the control group. The pyknosis was severe as well, even some nuclei of the outer nuclear layer dissolved. These results indicated that TCS exposure may have potential toxic ef-fects on eye development of zebrafish larvae. This provides a scientific basis for further exploring the poten-tial toxic effects of TCS on human body.

关 键 词：三氯生(TCS) 斑马鱼 眼 感光细胞 透射电镜(TEM) 

分 类 号：Q955[生物学—动物学] X171.5[环境科学与工程—环境科学]