广西北部湾来源抗菌活性真菌菌株的分离筛选及活性产物鉴定  

The isolation,screening and the active metabolites identification of bioactive fungal strains with antimicrobial activity from samples collected in the Guangxi Beibu Gulf region

作　　者：王聪 苏艳[2] 高谕康 谭学才 雷福厚 李浩 杜方凯 彭晓娉[3] 李小燕 WANG Cong;SU Yan;GAO Yu-kang;TAN Xue-cai;LEI Fu-hou;LI Hao;DU Fang-kai;PENG Xiao-ping;LI Xiao-yan(Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Guangxi Colleges and Universities forFood Safety and Pharmaceutical Analytical Chemistry,School of Chemistry and Chemical Engineering,Guangxi University forNationalities,Nanning 530006,China;Pharmaceutical and Bio-engineering Department,Zibo Vocational Institute,Zibo 255314,China;Department of Natural Medicinal Chemistry and Pharmacognosy,School of Pharmacy,Qingdao University,Qingdao 266021,China)

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西高校食品安全与药物分析化学重点实验室,南宁530006 [2]淄博职业学院制药与生物工程系,淄博255314 [3]青岛大学药学院,天然药物与生药学系,青岛266021

出　　处：《国际药学研究杂志》2019年第4期270-276,共7页Journal of International Pharmaceutical Research

基　　金：广西民族大学青年基金项目(2018MDQN003);广西民族大学引进人才科研启动项目(2017KJQD004);2018年广西高校中青年教师基础能力提升项目(2018KY0177);广西科技基地和人才专项(桂科AD18126005);广西创新驱动发展重大专项子课题(桂科AA17204087-21);广西自然科学基金项目(2017GXNSFBA198013,2017GXNSFBA198026);国家自然科学基金资助项目(40218030034)

摘　　要：目的从采自广西北部湾的泥样和植物中分离海洋真菌,筛选具有抗菌活性的真菌菌株,分离鉴定活性化合物。方法采用普通稀释法从样品中分离菌株,对次级代谢产物进行抑菌活性测试,以活性为导向分离抗菌活性化合物,并通过波谱方法确定化合物的结构。结果从采自广西北部湾的2个海泥和8个植物样品中分离72株真菌,筛选得到对香蕉枯萎病菌(Fusarium oxysporum f. sp cubense)、白色念珠菌(Candida albicans)、金黄色葡萄球菌(Staphylococcus aureus)和耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)等致病菌有抑菌作用的活性菌株13株,并从其中的1株真菌MDCW-15次级代谢产物中分离鉴定了烟曲霉素。抑菌活性测试结果显示,该化合物对白色念珠菌的最小抑菌浓度为64 μg/ml。结论从广西北部湾分离的药用活性真菌资源具有深入研究的价值。Objective To obtain bioactive fungal strains with antimicrobial activity from samples and plants collected in the Guangxi Beibu Gulf region,and identify the active metabolites. Methods The isolation of fungal strains was performed by the dilution method. The antimicrobial activity was assayed for the fungal strains using culture extracts to obtain bioactive fungal strains. The separation of antibacterial metabolites was carried out in a bioassay-guided manner. The structure of obtained compound was identified by the spectroscopic method. Results Seventy- two fungal strains were isolated from the soil and plant samples collected in the Guangxi Beibu Gulf region. Among them,13 strains exhibited antimicrobial activities on Fusarium oxysporum f. sp cubense,Candida albicans,Staphylococcus aureus or methicillin- resistant Staphylococcus aureus(MRSA). Fumagillin was isolated and identified from the secondary metabolites of one of the 13 fungal strains,MDCW-15. In the present bioassay,fumagillin displayed antifungal activity against C. albicans with the minimum inhibitory concentration(MIC)of 64.0 μg/ml. Conclusion Fungi from the Guangxi Beibu Gulf region are potential resources of antimicrobial fungal strains,whose active metabolites is worth further investigation.

关 键 词：广西北部湾 海洋真菌 抑菌活性 烟曲霉素 

分 类 号：R916[医药卫生—药学]