SALL4对白血病HL-60细胞放射敏感性影响研究  

Effect of SALL4 on radiosensitivity of leukemia cell line HL-60

作　　者：李琳[1] 吴广银[2] 李晓可 田丽晓[1] 陈玉清[3] 李艳[4] Li Lin;Wu Guangyin;Li Xiaoke;Tian Lixiao;Chen Yuqing;Li Yan(Department of Acute and Critical Medicine, Henan Provincial People′s Hospital, Zhengzhou 450003, China;Department of Radiation Oncology, Henan Provincial People′s Hospital, Zhengzhou 450003, China;Department of Hematology, Henan Provincial People′s Hospital, Zhengzhou 450003, China;Medical Imaging Center, Henan Provincial People′s Hospital, Zhengzhou 450003, China)

机构地区：[1]河南省人民医院急危重症医学部,郑州450003 [2]河南省人民医院放疗科,郑州450003 [3]河南省人民医院血液科,郑州450003 [4]河南省人民医院医学影像中心,郑州450003

出　　处：《中华放射肿瘤学杂志》2019年第9期706-708,共3页Chinese Journal of Radiation Oncology

摘　　要：目的探讨下调SALL4对白血病细胞放射敏感性影响,为提高白血病患者放射敏感性提供新思路。方法人急性髓系白血病细胞株HL-60感染shRNA SALL4和shRNA control慢病毒记为Lv-shSALL4组和Lv-shNC组,用RT-PCR和蛋白印迹法检测细胞中SALL4水平,同时取感染后的细胞给予8 Gy剂量照射处理记为Lv-shSALL4+放射组、Lv-shNC+放射组,流式细胞术检测细胞凋亡,蛋白印迹法检测细胞中Cleaved Caspase-3、Cleaved Caspase-9、Bax蛋白水平。取Lv-shSALL4组和Lv-shNC组细胞,给予0、2、4、6、8 Gy照射,细胞克隆形成实验测定放射增敏比。结果Lv-shSALL4组细胞中SALL4水平低于Lv-shNC组(P<0.05)。细胞凋亡率升高,细胞中Cleaved Caspase-3、Cleaved Caspase-9、Bax蛋白水平升高,与Lv-shNC组比较,差异有统计学意义(P<0.05)。Lv-shSALL4+放射组细胞增殖能力降低,细胞凋亡率升高,细胞中Cleaved Caspase-3、Cleaved Caspase-9、Bax蛋白水平升高(P<0.05)。Lv-shSALL4组细胞放射增敏比为1.323。结论下调SALL4增加白血病细胞放射敏感性,抑制白血病细胞增殖并诱导凋亡。Objective To evaluate the effect of down-regulating SALL4 on the radiosensitivity of leukemia cells, aiming to provide new ideas for improving radiosensitivity of leukemia patients. Methods Human acute myeloid leukemia cell line HL-60 infected with shRNA SALL4 and shRNA control lentivirus was classified into the Lv-shSALL4 group and Lv-shNC group. The levels of SALL4 mRNA and protein in cells were detected by RT-PCR and Western blot. The infected cells treated with 8 Gy dose irradiation were assigned into the Lv-shSALL4+ radiation and Lv-shNC+ radiation groups. The cell apoptosis was detected by flow cytometry. The levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins in cells were determined by Western blot. The cells in the Lv-shSALL4 and Lv-shNC groups were exposed to 0, 2, 4, 6 and 8 Gy irradiation. The radiosensitivity ratio was determined by cell clone test. Results The level of SALL4 in the Lv-shSALL4 group was significantly lower than that in the Lv-shNC group (P<0.05). The cell apoptosis rate was significantly increased, the levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins were remarkably up-regulated in cells compared with those in the Lv-shNC group (all P<0.05). The cell proliferation ability in the Lv-shSALL4+ radiation was significantly reduced, the cell apoptosis rate was considerably increased, the levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins were significantly up-regulated compared with those in the Lv-shSALL4 and Lv-shNC+ radiation groups (all P<0.05). The cell radiosensitization ratio in the Lv-shSALL4 group was 1.323. Conclusion Down-regulating SALL4 can increase the radiosensitivity of leukemic cells, inhibit the cell proliferation and induce the apoptosis of leukemic cells.

关 键 词：SALL4基因 HL-60细胞系 放射敏感性 

分 类 号：R730.55[医药卫生—肿瘤] R733.7[医药卫生—临床医学]