姜黄素-PLGA纳米粒的制备及诱导胶质瘤细胞凋亡的实验研究  

Study on preparation of curcumin-PLGA nanoparticles and their effect on apoptosis in glioma cells

作　　者：胡兴华 张峻槐 黄礼义 徐忠烨[1] HU Xing-hua;ZHANG Jun-huai;HUANG Li-yi;XU Zhong-ye(Dept of Neurosurgery,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;Dept of Rehabilitation,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第二医院神经外科,重庆400010 [2]重庆医科大学附属第二医院康复医学科,重庆400010

出　　处：《中国药理学通报》2019年第10期1399-1405,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年基金资助项目(No 81471676);重庆市科委前沿与应用基础研究(一般)项目(No cstc2014jcyjA10050);重庆市卫生局中医药课题(No ZY20132103);重庆市卫生局课题(No 2012-2-065)

摘　　要：目的制备姜黄素-PLGA纳米粒(Cur-PLGA-NPs),评价其对胶质瘤细胞的影响。方法C6、U251细胞传代培养,随机分为对照组、姜黄素组和姜黄素-PLGA组。倒置荧光显微镜、透射电镜观察Cur-PLGA-NPs的形态;马尔文粒径电位仪检测Cur-PLGA-NPs的粒径;酶标仪检测Cur-PLGA-NPs的包封率和载药率;荧光显微镜观察C6、U251细胞对Cur-PLGA-NPs的摄取;CCK-8检测Cur-PLGA-NPs对C6、U251细胞活性的影响;流式细胞术检测Cur-PLGA-NPs对凋亡的影响;Hoechst染色检测凋亡。结果Cur-PLGA-NPs的平均粒径为(284.6±9.0)nm,呈球形。其包封率为(70.712±2.615)%,载药率为(2.828±0.105)%。与对照组、姜黄素组相比,C6、U251细胞对Cur-PLGA-NPs的摄取均明显增加(P<0.05);与对照组、姜黄素组相比,姜黄素-PLGA组的C6、U251细胞活性均明显降低(P<0.05)。与对照组、姜黄素组相比,Cur-PLGA-NPs能更明显引起C6、U251细胞凋亡(P<0.05)。结论与姜黄素相比,Cur-PLGA-NPs能增强肿瘤细胞对药物的摄取,促进肿瘤细胞凋亡。Aim To prepare curcumin-PLGA nanoparticles[curcumin poly(lactic-co-glycolic acid) nanoparticles,Cur-PLGA-NPs] and evaluate their effects on glioma cells in vitro.Methods C6 cells and U251 cells were subcultured and randomly divided into control group,curcumin group and curcumin-PLGA group.The morphology of Cur-PLGA-NPs was observed by inverted fluorescence microscope and transmission electron microscopy.The particle size of Cur-PLGA-NPs was detected by Malvern particle size potentiometer.The encapsulation efficiency and drug loading rate of Cur-PLGA-NPs were detected by a multi-function microplate reader.The uptake of Cur-PLGA-NPs by C6 cells and U251 cells was observed by fluorescence microscopy.The effect of Cur-PLGA-NPs on the viability of C6 cells and U251 cells was detected by CCK-8.The effect of Cur-PLGA-NPs on apoptosis was assessed by flow cytometry.Apoptosis was detected by Hoechst staining.Results The Cur-PLGA-NPs had an average particle size of(284.6±9.0) nm and were spherical.The encapsulation efficiency was( 70.712 ± 2.615 )%,and the drug loading rate was( 2.828 ± 0.105 )%.Compared with control group and curcumin group,C6 cells and U251 cells significantly increased the uptake of Cur-PLGA-NPs( P <0.05).Compared with control group and curcumin group,the cell viability of C6 cells and U251 cells in curcumin-PLGA group significantly decreased( P <0.05).Compared with control group and curcumin group,the Cur-PLGA-NPs induced apoptosis significantly in C6 cells and U251 cells( P <0.05).Conclusion Compared with curcumin,Cur-PLGA-NPs can enhance the uptake of drugs by tumor cells,promoting tumor cell apoptosis.

关 键 词：姜黄素 胶质瘤 凋亡 PLGA C6细胞 U251细胞 

分 类 号：R-332[医药卫生] R282.71