三维联合培养牙髓细胞和血管内皮祖细胞促进成牙本质向/成骨向分化  

Three-dimensional Coculture of Dental Pulp Cells and Endothelial Progenitor Cells Enhances Osteo/odontogenic Differentiation

作　　者：陈婷[1,2,3] 侯晋 李心竹[1,3] 宋词[1,3] 张雪洋 吴补领 CHEN Ting;HOU Jin;LI Xin-zhu;SONG Ci;ZHANG Xue-yang;WU Bu-ling(Department of Stomatology, Nan fang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China;Shundc Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, Guangdong, 528300. China;School of Stomatology. Southern Medical University, Guangzhou, Guangdong, 510515, China)

机构地区：[1]南方医科大学南方医院口腔科,广东广州510515 [2]南方医科大学顺德医院(佛山市顺德区第一人民医院),广东佛山528300 [3]南方医科大学口腔医学院,广东广州510515

出　　处：《现代生物医学进展》2019年第16期3028-3032,3054,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81800950;81870755;81670986)

摘　　要：目的:探讨三维联合培养牙髓细胞(dental pulp cells, DPCs)和血管内皮祖细胞(endothelial progenitor cells, EPCs)对成牙本质向/成骨向分化的影响。方法:取单独培养DPCs及联合培养的DPCs和EPCs进行三维培养后成牙本质向/成骨向诱导,使用茜素红染色及半定量分析、RT-PCR和细胞免疫荧光检测成牙本质向/成骨向分化能力。采用SPSS 23.0统计软件对数据进行统计学分析。结果:茜素红染色显示联合培养组和单独培养组之间未见显著差异。RT-PCR和细胞免疫荧光显示成牙本质向/成骨向相关基因m RNAs和蛋白表达水平联合培养组显著高于单独培养组。结论:三维联合培养的DPCs和EPCs促进成牙本质向/成骨向分化,为牙髓再生提供可能实验依据。Objective: To observe the effect of three-dimensional coculture of dental pulp cells(DPCs) and endothelial progenitor cells(EPCs) on the osteo/odontogenic differentiation. Methods: After osteo/odontogenic induced of three-dimensional culture of the coculture of DPCs and EPCs and the culture of DPCs, Alizarin red stain, RT-PCR and immunofluorescence were used to detect the osteo/odontogenic differentiation. The data was analyzed using SPSS 23.0 software package. Results: Alizarin red stain showed that it is no significant differences between the coculture group and the culture group. RT-PCR and immunofluorescence showed that the coculture group is significant higher than the culture group on the m RNA and protein level of osteo/odontogenic differentiation.Conclusions: Three-dimensional coculture of dental pulp cells and endothelial progenitor cells enhances osteo/odontogenic differentiation.

关 键 词：牙髓细胞 血管内皮祖细胞 三维联合培养 成牙本质向/成骨向分化 

分 类 号：R-33[医药卫生] Q813[生物学—生物工程]